Document Detail


Troponin T3 expression in skeletal and smooth muscle is required for growth and postnatal survival: characterization of Tnnt3(tm2a(KOMP)Wtsi) mice.
MedLine Citation:
PMID:  23775847     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The troponin complex, which consists of three regulatory proteins (troponin C, troponin I, and troponin T), is known to regulate muscle contraction in skeletal and cardiac muscle, but its role in smooth muscle remains controversial. Troponin T3 (TnnT3) is a fast skeletal muscle troponin believed to be expressed only in skeletal muscle cells. To determine the in vivo function and tissue-specific expression of Tnnt3, we obtained the heterozygous Tnnt3+/flox/lacZ mice from Knockout Mouse Project (KOMP) Repository. Tnnt3(lacZ/+) mice are smaller than their WT littermates throughout development but do not display any gross phenotypes. Tnnt3(lacZ/lacZ) embryos are smaller than heterozygotes and die shortly after birth. Histology revealed hemorrhagic tissue in Tnnt3(lacZ/lacZ) liver and kidney, which was not present in Tnnt3(lacZ/+) or WT, but no other gross tissue abnormalities. X-gal staining for Tnnt3 promoter-driven lacZ transgene expression revealed positive staining in skeletal muscle and diaphragm and smooth muscle cells located in the aorta, bladder, and bronchus. Collectively, these findings suggest that troponins are expressed in smooth muscle and are required for normal growth and breathing for postnatal survival. Moreover, future studies with this mouse model can explore TnnT3 function in adult muscle function using the conditional-inducible gene deletion approach
Authors:
Yawen Ju; Jie Li; Chao Xie; Christopher T Ritchlin; Lianping Xing; Matthew J Hilton; Edward M Schwarz
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2013-07-09
Journal Detail:
Title:  Genesis (New York, N.Y. : 2000)     Volume:  51     ISSN:  1526-968X     ISO Abbreviation:  Genesis     Publication Date:  2013 Sep 
Date Detail:
Created Date:  2013-09-30     Completed Date:  2014-03-28     Revised Date:  2014-09-02    
Medline Journal Info:
Nlm Unique ID:  100931242     Medline TA:  Genesis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  667-75     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 Wiley Periodicals, Inc.
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MeSH Terms
Descriptor/Qualifier:
Animals
Kidney / abnormalities,  growth & development
Liver / abnormalities,  growth & development
Mice
Mice, Inbred C57BL
Mice, Knockout
Muscle, Skeletal / growth & development,  metabolism
Muscle, Smooth / growth & development,  metabolism*
Phenotype
Transgenes
Troponin
Troponin T / genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
AI078907/AI/NIAID NIH HHS; AR048697/AR/NIAMS NIH HHS; AR054041/AR/NIAMS NIH HHS; AR056702/AR/NIAMS NIH HHS; AR061307/AR/NIAMS NIH HHS; P30 AR061307/AR/NIAMS NIH HHS; R01 AR056702/AR/NIAMS NIH HHS; R01 AR057022/AR/NIAMS NIH HHS
Chemical
Reg. No./Substance:
0/Troponin; 0/Troponin T; 0/troponin T3, skeletal, fast, mouse
Comments/Corrections

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