Document Detail


Tropomyosin isoforms in nonmuscle cells.
MedLine Citation:
PMID:  9002235     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Vertebrate nonmuscle cells, such as human and rat fibroblasts, express multiple isoforms of tropomyosin, which are generated from four different genes and a combination of alternative promoter activities and alternative splicing. The amino acid variability among these isoforms is primarily restricted to three alternatively spliced exon regions; an amino-terminal region, an internal exon, and a carboxyl-terminal exon. Recent evidence reveals that these variable exon regions encode amino acid sequences that may dictate isoform-specific functions. The differential expression of tropomyosin isoforms found in cell transformation and cell differentiation, as well as the differential localization of tropomyosin isoforms in some types of culture cells and developing neurons suggest a differential isoform function in vivo. Tropomyosin in striated muscle works together with the troponin complex to regulate muscle contraction in a Ca(2+)-dependent fashion. Both in vitro and in vivo evidence suggest that multiple isoforms of tropomyosin in nonmuscle cells may be required for regulating actin filament stability, intracellular granule movement, cell shape determination, and cytokinesis. Tropomyosin-binding proteins such as caldesmon, tropomodulin, and other unidentified proteins may be required for some of these functions. Strong evidence for the distinct functions carried out by different tropomyosin isoforms has been generated from genetic analysis of yeast and Drosophila tropomyosin mutants.
Authors:
J J Lin; K S Warren; D D Wamboldt; T Wang; J L Lin
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.; Review    
Journal Detail:
Title:  International review of cytology     Volume:  170     ISSN:  0074-7696     ISO Abbreviation:  Int. Rev. Cytol.     Publication Date:  1997  
Date Detail:
Created Date:  1997-02-20     Completed Date:  1997-02-20     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985180R     Medline TA:  Int Rev Cytol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1-38     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, University of Iowa, Iowa City 52242-1324, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Fibroblasts / chemistry*,  physiology
Humans
Isoenzymes / chemistry*,  physiology
Tropomyosin / chemistry*,  physiology
Grant Support
ID/Acronym/Agency:
HD 18577/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Isoenzymes; 0/Tropomyosin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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