| Trophoblasts and shear stress induce an asymmetric distribution of icam-1 in uterine endothelial cells. | |
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MedLine Citation:
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PMID: 18211542 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PROBLEM: We have used an in vitro co-culture system consisting of early gestation macaque trophoblasts cultured on top of human uterine microvascular endothelial cells (UtMVECs) to investigate the inflammatory response of endothelial cells to trophoblasts under shear stress conditions. METHOD: of study Uterine microvascular endothelial cells and trophoblasts were co-cultured in a parallel plate chamber under shear stress (15 dyn/cm(2)) conditions. The distribution and expression of endothelial intercellular adhesion molecule-1 (ICAM-1) was quantified by immunofluorescence image analysis and flow cytometry. Endothelial regulated upon activation normal T-cell expressed and secreted (RANTES) secretion was measured by enzyme-linked immunosorbent assay and permeability was assessed using fluorescein isothiocyanate-dextran. RESULTS: Intercellular adhesion molecule-1, but not vascular cell adhesion molecule-1 or platelet endothelial cell adhesion molecule-1, was re-distributed towards the downstream edge of endothelial cells when the cells were co-cultured with trophoblasts under shear stress conditions. Changes in ICAM-1 distribution were also observed when UtMVECs were co-cultured with trophoblast-conditioned medium under shear stress conditions. Incubation of UtMVECs with trophoblast-conditioned medium increased endothelial permeability, RANTES secretion, and trophoblast adhesion. CONCLUSION: These data support the idea that trophoblasts induce an inflammatory response in uterine endothelial cells that could enhance trophoblast invasion and transmigration. |
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Authors:
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Tim C Cao; Twanda L Thirkill; Mark Wells; Abdul I Barakat; Gordon C Douglas |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: American journal of reproductive immunology (New York, N.Y. : 1989) Volume: 59 ISSN: 1046-7408 ISO Abbreviation: Am. J. Reprod. Immunol. Publication Date: 2008 Feb |
Date Detail:
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Created Date: 2008-01-23 Completed Date: 2008-04-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8912860 Medline TA: Am J Reprod Immunol Country: Denmark |
Other Details:
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Languages: eng Pagination: 167-81 Citation Subset: IM |
Affiliation:
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Department of Biomedical Engineering, University of California, Davis, CA 95616, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Adhesion / immunology Cell Movement / immunology Chemokine CCL5 / immunology Coculture Techniques Endothelial Cells / cytology, immunology Female Humans Immunohistochemistry Intercellular Adhesion Molecule-1 / immunology* Macaca mulatta Microscopy, Fluorescence Microscopy, Phase-Contrast Pregnancy Stress, Mechanical Trophoblasts / cytology, immunology* Uterus / cytology, immunology* |
| Grant Support | |
ID/Acronym/Agency:
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R01HL068035-01A1/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Chemokine CCL5; 126547-89-5/Intercellular Adhesion Molecule-1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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