| Trophoblasts isolated from the maternal circulation: in vitro expansion and potential application in non-invasive prenatal diagnosis. | |
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MedLine Citation:
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PMID: 15750014 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Prenatal diagnosis based on rare fetal cells in maternal blood is currently not a feasible option. An effort was made to improve cell yields by targeting trophoblast cells. After sorting, the HLA-G-positive cell fraction was analyzed directly or after culture. In situ hybridization technology was applied to prove fetal cell source in samples from women carrying a male fetus and to predict gender in samples without previous knowledge of fetal sex. In vitro culture led to a significant increase in fetal cells and accurate gender prediction in 93% of these samples. This approach might be useful for non-invasive prenatal diagnosis. |
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Authors:
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Esther Guetta; Liat Gutstein-Abo; Gad Barkai |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society Volume: 53 ISSN: 0022-1554 ISO Abbreviation: J. Histochem. Cytochem. Publication Date: 2005 Mar |
Date Detail:
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Created Date: 2005-03-07 Completed Date: 2005-04-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9815334 Medline TA: J Histochem Cytochem Country: United States |
Other Details:
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Languages: eng Pagination: 337-9 Citation Subset: IM |
Affiliation:
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Danek Gertner Institute of Human Genetics, Sheba Medical Center, Tel-Hashomer, Israel 52621. Esther.Guetta@sheba.health.gov.il |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Centrifugation, Density Gradient Female HLA Antigens / metabolism Histocompatibility Antigens Class I / metabolism Humans Immunomagnetic Separation* In Situ Hybridization, Fluorescence Male Pregnancy Prenatal Diagnosis / methods Sex Determination (Analysis) / methods Trophoblasts / cytology*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/HLA Antigens; 0/HLA-G antigen; 0/Histocompatibility Antigens Class I |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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