Document Detail

Trophoblasts isolated from the maternal circulation: in vitro expansion and potential application in non-invasive prenatal diagnosis.
MedLine Citation:
PMID:  15750014     Owner:  NLM     Status:  MEDLINE    
Prenatal diagnosis based on rare fetal cells in maternal blood is currently not a feasible option. An effort was made to improve cell yields by targeting trophoblast cells. After sorting, the HLA-G-positive cell fraction was analyzed directly or after culture. In situ hybridization technology was applied to prove fetal cell source in samples from women carrying a male fetus and to predict gender in samples without previous knowledge of fetal sex. In vitro culture led to a significant increase in fetal cells and accurate gender prediction in 93% of these samples. This approach might be useful for non-invasive prenatal diagnosis.
Esther Guetta; Liat Gutstein-Abo; Gad Barkai
Related Documents :
6602774 - Studies on thymocyte subpopulations in guinea pigs. 2. three subpopulations differing i...
25264874 - Aligned and suspended fiber force probes for drug testing at single cell resolution.
9252694 - Detection of rare human breast cancer cells. comparison of an immunomagnetic separation...
8423364 - An improved mtt assay.
9371524 - Morphology of acute promyelocytic leukemia with cytogenetic or molecular evidence for t...
7166534 - Increase of cardiolipin content in staphylococcus aureus by the use of antibiotics affe...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  53     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-03-07     Completed Date:  2005-04-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  337-9     Citation Subset:  IM    
Danek Gertner Institute of Human Genetics, Sheba Medical Center, Tel-Hashomer, Israel 52621.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Centrifugation, Density Gradient
HLA Antigens / metabolism
Histocompatibility Antigens Class I / metabolism
Immunomagnetic Separation*
In Situ Hybridization, Fluorescence
Prenatal Diagnosis / methods
Sex Determination (Analysis) / methods
Trophoblasts / cytology*,  metabolism
Reg. No./Substance:
0/HLA Antigens; 0/HLA-G antigen; 0/Histocompatibility Antigens Class I

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The fetal erythroblast is not the optimal target for non-invasive prenatal diagnosis: preliminary re...
Next Document:  Proteome analysis of maternal serum samples for trisomy 21 pregnancies using ProteinChip arrays and ...