Document Detail


Trophoblasts isolated from the maternal circulation: in vitro expansion and potential application in non-invasive prenatal diagnosis.
MedLine Citation:
PMID:  15750014     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Prenatal diagnosis based on rare fetal cells in maternal blood is currently not a feasible option. An effort was made to improve cell yields by targeting trophoblast cells. After sorting, the HLA-G-positive cell fraction was analyzed directly or after culture. In situ hybridization technology was applied to prove fetal cell source in samples from women carrying a male fetus and to predict gender in samples without previous knowledge of fetal sex. In vitro culture led to a significant increase in fetal cells and accurate gender prediction in 93% of these samples. This approach might be useful for non-invasive prenatal diagnosis.
Authors:
Esther Guetta; Liat Gutstein-Abo; Gad Barkai
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  53     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-03-07     Completed Date:  2005-04-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  337-9     Citation Subset:  IM    
Affiliation:
Danek Gertner Institute of Human Genetics, Sheba Medical Center, Tel-Hashomer, Israel 52621. Esther.Guetta@sheba.health.gov.il
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MeSH Terms
Descriptor/Qualifier:
Centrifugation, Density Gradient
Female
HLA Antigens / metabolism
Histocompatibility Antigens Class I / metabolism
Humans
Immunomagnetic Separation*
In Situ Hybridization, Fluorescence
Male
Pregnancy
Prenatal Diagnosis / methods
Sex Determination (Analysis) / methods
Trophoblasts / cytology*,  metabolism
Chemical
Reg. No./Substance:
0/HLA Antigens; 0/HLA-G antigen; 0/Histocompatibility Antigens Class I

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