Document Detail

Trophoblast CD274 (B7-H1) is differentially expressed across gestation: influence of oxygen concentration.
MedLine Citation:
PMID:  16251499     Owner:  NLM     Status:  MEDLINE    
Modulation of the maternal immune system by the placenta is a mechanism by which the fetus ensures its own survival in a genetically foreign environment. The immunoinhibitor CD274 (also called B7-H1 or PD-L1) is highly expressed in the placenta, positioned to interact with maternal leukocytes. Further, immunoblot analysis of first- and second-trimester placental lysates showed that CD274 expression is low in the first trimester but dramatically rises around the onset of the second trimester. As this coincides with the expected onset of maternal blood flow to the placenta and a corresponding rise in local oxygen tension, we explored the possibility that oxygen regulates CD274 expression in trophoblast cells by culturing term trophoblast cells under oxygen concentrations similar to those found in vivo. Indeed, CD274 protein levels paralleled the in vivo situation: expression increased with rising oxygen concentrations. Furthermore, downregulation of CD274 mRNA by low oxygen was rapid, occurring within 4-12 h. We conclude that oxygen is a potential mediator of CD274 expression in vivo such that it is induced coincidentally on exposure of fetal tissues to maternal blood. Further, the regulation of this immunomodulator by oxygen may implicate its alteration during and involvement in the pathogenesis of complications of pregnancy such as preeclampsia.
Lesya M Holets; Joan S Hunt; Margaret G Petroff
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2005-10-26
Journal Detail:
Title:  Biology of reproduction     Volume:  74     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-01-26     Completed Date:  2006-04-10     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  352-8     Citation Subset:  IM    
Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, Kansas 66160, USA.
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MeSH Terms
Antigens, CD
Antigens, CD80 / genetics,  metabolism*
Cells, Cultured
Gestational Age
Membrane Glycoproteins / genetics,  metabolism*
Oxygen / metabolism*
Peptides / genetics,  metabolism*
Placenta / cytology,  immunology,  metabolism*
Pregnancy Trimesters
RNA, Messenger / metabolism
Trophoblasts / metabolism*
Grant Support
Reg. No./Substance:
0/Antigens, CD; 0/Antigens, CD80; 0/CD274 protein, human; 0/Membrane Glycoproteins; 0/Peptides; 0/RNA, Messenger; 7782-44-7/Oxygen

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