Document Detail


Trityl-based EPR probe with enhanced sensitivity to oxygen.
MedLine Citation:
PMID:  19523513     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An asymmetric derivative of the triarylmethyl radical, TAM-H, containing one aldehyde and two carboxyl groups, was synthesized. The electron paramagnetic resonance (EPR) spectrum of TAM-H is characterized by a doublet of narrow lines with a linewidth of 105 mG under anoxic conditions and hyperfine interaction constant of 245 mG. The partial overlap of the components of the doublet results in enhanced sensitivity of the spectral amplitudes ratio to oxygen compared with oxygen-induced linewidth broadening of a single line. Application of the TAM-H probe allows for EPR measurements in an extended range of oxygen pressures from atmospheric to 1 mm Hg, whereas the EPR spectrum linewidth of the popular TAM-based oxygen sensor Oxo63 is practically insensitive to oxygen partial pressures below 20 mm Hg. Enhanced sensitivity of the TAM-H probe relative to Oxo63 was demonstrated in the detection of oxygen consumption by Met-1 cancer cells. The TAM-H probe allowed prolonged measurements of oxygen depletion during the hypoxia stage and down to true anoxia (<or=1.5 mm Hg).
Authors:
Andrey A Bobko; Ilirian Dhimitruka; Timothy D Eubank; Clay B Marsh; Jay L Zweier; Valery V Khramtsov
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural     Date:  2009-06-10
Journal Detail:
Title:  Free radical biology & medicine     Volume:  47     ISSN:  1873-4596     ISO Abbreviation:  Free Radic. Biol. Med.     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-07-27     Completed Date:  2009-12-07     Revised Date:  2014-09-21    
Medline Journal Info:
Nlm Unique ID:  8709159     Medline TA:  Free Radic Biol Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  654-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Atmospheric Pressure
Biosensing Techniques
Cells, Cultured
Electron Spin Resonance Spectroscopy / methods
Free Radicals / analysis,  metabolism
Mice
Models, Biological
Oxygen / metabolism,  pharmacology*
Sensitivity and Specificity
Spin Labels / chemical synthesis*
Trityl Compounds / chemical synthesis*,  chemistry,  pharmacology*
Grant Support
ID/Acronym/Agency:
EB00890/EB/NIBIB NIH HHS; EB0490/EB/NIBIB NIH HHS; K01 EB003519/EB/NIBIB NIH HHS; K01 EB003519-05/EB/NIBIB NIH HHS; KO1 EB03519/EB/NIBIB NIH HHS; R01 EB000890/EB/NIBIB NIH HHS; R01 EB000890-04/EB/NIBIB NIH HHS; R21 CA132068/CA/NCI NIH HHS; R21 CA132068/CA/NCI NIH HHS; R21 CA132068-02/CA/NCI NIH HHS; R21 HL091423/HL/NHLBI NIH HHS; R21 HL091423-01/HL/NHLBI NIH HHS; R21 HL091423-02/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Free Radicals; 0/Spin Labels; 0/Trityl Compounds; S88TT14065/Oxygen
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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