| Tritrichomonas foetus: characterization of isolates and partial purification of a secreted cytotoxin. | |
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MedLine Citation:
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PMID: 12615161 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Putative virulence factors including extracellular proteases, hemagglutinin, hemolysins, and soluble cytotoxins may play significant roles in the pathogenesis of trichomoniasis. The cytotoxicity, hemagglutinating, and hemolytic activity of Tritrichomonas foetus isolate ATCC #30003 and several field isolates were compared. All isolates were hemolytic toward mouse and bovine erythrocytes but not other tested species. The isolates varied significantly in hemagglutinating ability and cytotoxin production. A 40,000 Da soluble cytotoxin was partially purified and characterized. Chromatography separated cytotoxic activity from hemagglutinating and hemolytic activity but not from protease activity. However, protease assays indicated that protease activity was inversely correlated with cytotoxic activity. Characterization studies indicated that cytotoxic activity was destroyed by heat and acidic conditions but repeated freeze/thawing did not diminish activity. Target cell specificity assays showed Henle cells were twice as sensitive to the effects of the cytotoxin as Vero cells. These results suggest that T. foetus isolates vary in the production of virulence factors and produce a soluble relatively stable non-protease cytotoxic protein capable of killing cultured mammalian cells in vitro. |
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Authors:
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Mary J Kennett; Reuel R Hook |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Experimental parasitology Volume: 102 ISSN: 0014-4894 ISO Abbreviation: Exp. Parasitol. Publication Date: 2002 Sep |
Date Detail:
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Created Date: 2003-03-04 Completed Date: 2003-04-15 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370713 Medline TA: Exp Parasitol Country: United States |
Other Details:
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Languages: eng Pagination: 1-8 Citation Subset: IM |
Copyright Information:
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Copyright 2003 Elsevier Science (USA) |
Affiliation:
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Department of Veterinary Science, College of Agricultural Sciences, The Pennsylvania State University, 101 Centralized Biological Laboratory, University Park, PA 16802, USA. mjk26@psu.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle Cell Line Cell Survival Cercopithecus aethiops Chromatography, Gel Chromatography, Ion Exchange Culture Media, Serum-Free Cytotoxins / biosynthesis, chemistry, isolation & purification*, toxicity Guinea Pigs Hemagglutination Tests Hemolysis Humans Hydrogen-Ion Concentration Mice Molecular Weight Rats Tritrichomonas foetus / pathogenicity* Ultrafiltration Vero Cells Virulence |
| Chemical | |
Reg. No./Substance:
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0/Culture Media, Serum-Free; 0/Cytotoxins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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