Document Detail


Triton X-100 as the "short-chain lipid" improves the magnetic alignment and stability of membrane proteins in phosphatidylcholine bilayers for oriented-sample solid-state NMR spectroscopy.
MedLine Citation:
PMID:  20735058     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A mixture of phospholipids and Triton X-100 in a molar ratio of 5:1 forms well-aligned and stable bilayers that give superior solid-state NMR spectra of proteins. In a comparison, the oriented-sample (OS) solid-state NMR spectrum of Pf1 coat protein in aligned phospholipid bilayers displayed better resolution than the equivalent solution NMR spectrum of the same protein in micelles. Both samples and experimental parameters were fully optimized.
Authors:
Sang Ho Park; Stanley J Opella
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Journal of the American Chemical Society     Volume:  132     ISSN:  1520-5126     ISO Abbreviation:  J. Am. Chem. Soc.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-09-08     Completed Date:  2011-01-05     Revised Date:  2014-09-17    
Medline Journal Info:
Nlm Unique ID:  7503056     Medline TA:  J Am Chem Soc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  12552-3     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Lipid Bilayers / chemistry*
Magnetic Resonance Spectroscopy
Magnetics*
Membrane Proteins / chemistry*
Octoxynol / chemistry*
Phosphatidylcholines / chemistry*
Grant Support
ID/Acronym/Agency:
P41 EB002031/EB/NIBIB NIH HHS; P41 EB002031-18/EB/NIBIB NIH HHS; R01 EB005161/EB/NIBIB NIH HHS; R01 EB005161-04S1/EB/NIBIB NIH HHS; R01 EB005161-05/EB/NIBIB NIH HHS; R01 GM075877/GM/NIGMS NIH HHS; R01 GM075877-05/GM/NIGMS NIH HHS; R01 GM075877-05S1/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Lipid Bilayers; 0/Membrane Proteins; 0/Phosphatidylcholines; 9002-93-1/Octoxynol
Comments/Corrections

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