Document Detail


Triterpenes augment the inhibitory effects of anticancer drugs on growth of human esophageal carcinoma cells in vitro and suppress experimental metastasis in vivo.
MedLine Citation:
PMID:  19462449     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The antineoplastic effects of combinations of anticancer drugs (5-fluorouracil, irinotecan and cisplatin) and triterpenes (ursolic acid, betulinic acid, oleanolic acid and a Japanese apricot extract (JAE) containing triterpenes) on esophageal squamous carcinoma cells were examined by the WST-8 (2-(2-methoxy- 4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt) assay in vitro and by an animal model in vivo. Triterpenes and JAE showed additive and synergistic cytotoxic effects, respectively, on esophageal squamous carcinoma cells (YES-2 cells) by combinational use of 5-fluorouracil. JAE and 5-fluorouracil induced cell cycle arrest at G2/M phase and at S phase, respectively, and caused apoptosis in YES-2 cells. A new animal model of esophageal cancer causing tumor colonization of the peritoneal cavity and producing bloody ascites was made by injecting YES-2 cells into the peritoneal cavity of a severe combined immunodeficiency mouse. In this model, 5-fluorouracil inhibited colonization of tumor cells in the peritoneum. The addition of JAE to 5-fluorouracil augmented the suppression of experimental metastasis of the peritoneum. The numbers of peritoneal nodules of more than 2 mm in diameter in mice treated with 5-fluorouracil and JAE were less than those in mice treated with 5-fluorouracil alone or JAE alone. These results suggest that triterpenes, especially JAE, are effective supplements for enhancing the chemotherapeutic effect of 5-fluorouracil on esophageal cancer.
Authors:
Hiromichi Yamai; Naruhiko Sawada; Takahiro Yoshida; Jyunichi Seike; Hiromitsu Takizawa; Koichiro Kenzaki; Takanori Miyoshi; Kazuya Kondo; Yoshimi Bando; Yoshinari Ohnishi; Akira Tangoku
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of cancer. Journal international du cancer     Volume:  125     ISSN:  1097-0215     ISO Abbreviation:  Int. J. Cancer     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-06-22     Completed Date:  2009-08-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0042124     Medline TA:  Int J Cancer     Country:  United States    
Other Details:
Languages:  eng     Pagination:  952-60     Citation Subset:  IM    
Affiliation:
Department of Thoracic, Endocrine Surgery and Oncology, Institute of Health Bioscience, The University of Tokushima Graduate School, Tokushima, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antimetabolites, Antineoplastic / pharmacology
Antineoplastic Agents, Phytogenic / pharmacology
Apoptosis / drug effects
Camptothecin / analogs & derivatives*,  pharmacology
Carcinoma, Squamous Cell / drug therapy*,  secondary
Cell Cycle / drug effects
Cell Line, Tumor
Cell Proliferation / drug effects*
Drug Interactions
Drug Synergism
Esophageal Neoplasms / drug therapy*,  pathology
Fluorouracil / pharmacology*
Humans
Male
Mice
Mice, Nude
Mice, SCID
Peritoneal Neoplasms / drug therapy*,  secondary
Plant Preparations / pharmacology
Prunus / chemistry
Triterpenes / pharmacology*
Xenograft Model Antitumor Assays
Chemical
Reg. No./Substance:
0/Antimetabolites, Antineoplastic; 0/Antineoplastic Agents, Phytogenic; 0/Plant Preparations; 0/Triterpenes; 100286-90-6/irinotecan; 51-21-8/Fluorouracil; 7689-03-4/Camptothecin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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