Document Detail

Trisomy 12 in chronic lymphoid leukemias--a metaphase and interphase cytogenetic analysis.
MedLine Citation:
PMID:  8464229     Owner:  NLM     Status:  MEDLINE    
Trisomy 12 has been shown to be one of the most common chromosome abnormalities in chronic lymphoid leukemias of B-cell origin, and some studies suggested that it predicts poor overall survival. We have prospectively studied 42 patients with B-cell chronic lymphocytic leukemia (B-CLL) and three patients with B-prolymphocytic leukemia (B-PLL) for the incidence of trisomy 12 and other chromosome 12 aberrations applying fluorescence in situ hybridization (ISH) and conventional G-banding analysis. Dual-color hybridization experiments using centromere-12-specific DNA probes were performed for interphase cytogenetics. A subset of patients (n = 11) was analyzed using a DNA library for painting of chromosome 12. The incidence of trisomy/partial trisomy 12 was 18% (8/45 patients; 6/42 with B-CLL and 2/3 with B-PLL) by fluorescence ISH, and 11% (5/45 patients; 4/42 with B-CLL including one patient with partial trisomy 12q13-qter, and 1/3 with B-PLL) on G-banding analysis. Four patients with trisomy 12 were detected by ISH alone. One of these patients only had 4.5% interphase cells with three fluorescence signals indicating the presence of a small subclone with trisomy 12. On G-banding analysis, three of the four patients had a normal karyotype, and one patient had no analyzable metaphases. In conclusion, fluorescence ISH to interphase nuclei is a sensitive method for detecting trisomy 12 in patients with chronic lymphoid leukemias.
H Döhner; S Pohl; M Bulgay-Mörschel; S Stilgenbauer; M Bentz; P Lichter
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Leukemia     Volume:  7     ISSN:  0887-6924     ISO Abbreviation:  Leukemia     Publication Date:  1993 Apr 
Date Detail:
Created Date:  1993-05-06     Completed Date:  1993-05-06     Revised Date:  2013-03-04    
Medline Journal Info:
Nlm Unique ID:  8704895     Medline TA:  Leukemia     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  516-20     Citation Subset:  IM    
Medizinische Klinik and Poliklinik V, Universität Heidelberg, Germany.
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MeSH Terms
Chromosome Banding
Chromosomes, Human, Pair 12*
DNA Probes
In Situ Hybridization, Fluorescence
Leukemia, Lymphocytic, Chronic, B-Cell / genetics*
Leukemia, Prolymphocytic / genetics*
Prospective Studies
Reg. No./Substance:
0/DNA Probes

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