Document Detail


Triptolide induced cytotoxic effects on human promyelocytic leukemia, T cell lymphoma and human hepatocellular carcinoma cell lines.
MedLine Citation:
PMID:  11397559     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Triptolide, a traditional Chinese medicine, has been reported to be effective in the treatment of auto-immune diseases, and it can also induce anti-neoplastic activity on several human tumor cell lines. This study investigates the cytotoxic function and the functional mechanism of triptolide on tumor cells. Promyelocytic leukemia, (HL-60), T cell lymphoma (Jurkat), and human hepatocelluar carcinoma (SMMC-7721) cells were subjected to triptolide treatment, and cell growth inhibition was examined by XTT cell viability assay. Cell death mechanism (apoptosis) was confirmed through DNA fragmentation and DAPI staining. Triptolide inhibited 50% of cell growth (IC(50)) on HL-60 cells at 7.5 nM, Jurkat cells at 27.5 nM and SMMC cells at 32 nM. Characteristic apoptotic features including internucleosomal DNA fragmentation and chromatin condensation were observed in triptolide treated cells. Data from the study indicates that triptolide could induce apoptosis in human tumor cell lines and it may be applicable as a potential chemotherapeutic agent for cancer treatment.
Authors:
E W Chan; S C Cheng; F W Sin; Y Xie
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Toxicology letters     Volume:  122     ISSN:  0378-4274     ISO Abbreviation:  Toxicol. Lett.     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-06-08     Completed Date:  2001-07-26     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7709027     Medline TA:  Toxicol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  81-7     Citation Subset:  IM    
Affiliation:
Department of Biology, The Hong Kong University of Science and Technology, Clearwater Bay, Kowloon, Hong Kong ROC.
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MeSH Terms
Descriptor/Qualifier:
Antineoplastic Agents, Alkylating / pharmacology*
Cell Survival / drug effects*
DNA Fragmentation / drug effects
Diterpenes / pharmacology*
Dose-Response Relationship, Drug
Epoxy Compounds
HL-60 Cells
Humans
Indoles
Jurkat Cells
Microscopy, Fluorescence
Phenanthrenes*
Staining and Labeling
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Antineoplastic Agents, Alkylating; 0/Diterpenes; 0/Epoxy Compounds; 0/Indoles; 0/Phenanthrenes; 38748-32-2/triptolide; 47165-04-8/DAPI

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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