| Triphenyltetrazolium chloride (TTC) as a marker for ischaemic changes in rat brain following permanent middle cerebral artery occlusion. | |
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MedLine Citation:
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PMID: 2057051 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Triphenyltetrazolium chloride (TTC) was used to delineate ischaemic lesions in the rat brain at various times following middle cerebral artery occlusion. A comparison was made of TTC staining by immersion and perfusion techniques and conventional light microscopy. The lesions were quantified by measuring the ischaemic area at the sections corresponding to 7 mm in front of the AO line (atlas of Konig and Klippel). In animals examined 24 h after middle cerebral artery occlusion (MCAO), the area of infarction was 17.4 +/- 1.3 mm2 on the TTC perfused slices and 17.6 +/- 1.6 mm2 on the TTC immersed slices (mean +/- SEM). By contrast, there was a marked difference between the two TTC methods when tissues were examined at shorter intervals after artery occlusion. In the TTC-perfused animals, there was no significant difference between the mean areas of infarction measured at 5-20 min, 3-4 h, or 24 h post occlusion. Immersion in TTC, however, failed to reveal any consistent ischaemic damage when applied at the earlier post-occlusion times. Conventional histopathology demonstrated minimal lesions at 5-20 min but at 4 h or more the lesions were not significantly different from those demonstrated by TTC perfusion. TTC immersion staining can, thus, only be used as a reliable marker of cerebral ischaemia damage with post-occlusion survival periods of 24 h. TTC perfusion staining gives results not significantly different from histopathology at 4 h or more post-occlusion but at earlier intervals than 24 h it differs significantly from TTC immersion staining.(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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R H Hatfield; A D Mendelow; R H Perry; L M Alvarez; P Modha |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Neuropathology and applied neurobiology Volume: 17 ISSN: 0305-1846 ISO Abbreviation: Neuropathol. Appl. Neurobiol. Publication Date: 1991 Feb |
Date Detail:
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Created Date: 1991-08-01 Completed Date: 1991-08-01 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7609829 Medline TA: Neuropathol Appl Neurobiol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 61-7 Citation Subset: IM |
Affiliation:
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Merck, Sharp and Dohme Research Laboratories, Neuroscience Research Centre, Harlow. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arterial Occlusive Diseases / pathology* Brain Ischemia / pathology* Cerebral Arteries / pathology* Histocytochemistry Male Perfusion Rats Rats, Inbred Strains Tetrazolium Salts / diagnostic use* Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Tetrazolium Salts; 902-00-1/triphenyltetrazolium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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