Document Detail


Triphasic and quadriphasic waveforms are superior to biphasic waveforms for synchronized beating of cardiomyocytes.
MedLine Citation:
PMID:  22336433     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
BACKGROUND AND PURPOSE: Within pacemaker research few attempts have been made to find an optimal waveform phase sequence that synchronizes beating of cardiomyocytes at an electrode. Multielectrode arrays (MEAs) offer electrophysiological screening of cardiomyocytes serving as a system for preliminary screening of pacing waveform design. MATERIALS AND METHODS: The HL-1 cell line was cultured in MEAs until confluence and stimulated with biphasic, triphasic, and quadriphasic waveforms. The amplitudes required for synchronized beating of the cells were determined. RESULTS: Triphasic and quadriphasic waveforms were more efficient in eliciting synchronized beating of the HL-1 cells compared with the biphasic waveform because it allows significant reductions in synchronizing voltage amplitudes and reductions in supplied stimulus. CONCLUSION: The MEA system allows for a straightforward manner to investigate effects of waveform design on synchronized beating in cardiomyocytes in vitro. Increased number of phase changes in a pacing waveform seems to be the major reason for the reduction in synchronizing amplitudes.
Authors:
Lars Enochson; Joakim Sandstedt; Hans Strandberg; Cecilia Emanuelsson; Andreas Ornberg; Anders Lindahl; Camilla Karlsson
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-2-13
Journal Detail:
Title:  Journal of electrocardiology     Volume:  -     ISSN:  1532-8430     ISO Abbreviation:  -     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-2-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0153605     Medline TA:  J Electrocardiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier Inc. All rights reserved.
Affiliation:
Department of Clinical Chemistry and Transfusion Medicine, The Sahlgrenska Academy, Gothenburg University, Gothenburg, Sweden; BIOMATCELL VINN Excellence Center of Biomaterials and Cell Therapy, Göteborg, Sweden.
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