Document Detail


Trinitrophenylation of rabbit skeletal myosin by 2,4,6-trinitrobenzene sulfonate and treatment of trinitrophenyl myosin with dithiothreitol.
MedLine Citation:
PMID:  2991209     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Rabbit skeletal myosin was trinitrophenylated with 2,4,6-trinitrobenzene sulfonate (TNBS) in the presence or absence of inorganic pyrophosphate (PP1). When myosin trinitrophenylated either in the presence or absence of PP1 was treated with dithiothreitol (DTT), the absorbance at 345 nm of both trinitrophenylated myosins was decreased, as though the trinitrophenyl groups bound to myosin were removed. The DTT treatment also essentially reversed the inhibition of the EDTA-ATPase and Ca-ATPase activities that was caused by trinitrophenylation of myosin. These effects of trinitrophenylation and of DTT treatment were independent of the presence or absence of PP1 during the trinitrophenylation. In contrast, the PP1-induced formation of a difference spectrum of trinitrophenylated myosin was not affected by the DTT treatment. On the basis of these observations, it is suggested that the "reactive lysine residues," trinitrophenylation of which resulted in inhibition of the ATPase activities, are different from those whose trinitrophenyl groups show an altered spectrum on addition of PP1.
Authors:
S Kodama; K Konno; K Arai; S Watanabe
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biochemistry     Volume:  97     ISSN:  0021-924X     ISO Abbreviation:  J. Biochem.     Publication Date:  1985 Mar 
Date Detail:
Created Date:  1985-09-04     Completed Date:  1985-09-04     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0376600     Medline TA:  J Biochem     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  831-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphatases / antagonists & inhibitors,  metabolism*
Animals
Binding Sites / drug effects
Calcium-Transporting ATPases / metabolism
Diphosphates / pharmacology*
Dithiothreitol / pharmacology*
Enzyme Activation / drug effects
Muscles / metabolism
Myosins / metabolism*
Nitrobenzenes*
Potassium Chloride / pharmacology
Rabbits
Trinitrobenzenesulfonic Acid*
Chemical
Reg. No./Substance:
0/Diphosphates; 0/Nitrobenzenes; 2508-19-2/Trinitrobenzenesulfonic Acid; 3483-12-3/Dithiothreitol; 7447-40-7/Potassium Chloride; EC 3.6.1.-/Adenosine Triphosphatases; EC 3.6.1.-/EDTA-ATPase; EC 3.6.1.8/Calcium-Transporting ATPases; EC 3.6.4.1/Myosins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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