| Trinitrophenylation of rabbit skeletal myosin by 2,4,6-trinitrobenzene sulfonate and treatment of trinitrophenyl myosin with dithiothreitol. | |
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MedLine Citation:
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PMID: 2991209 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Rabbit skeletal myosin was trinitrophenylated with 2,4,6-trinitrobenzene sulfonate (TNBS) in the presence or absence of inorganic pyrophosphate (PP1). When myosin trinitrophenylated either in the presence or absence of PP1 was treated with dithiothreitol (DTT), the absorbance at 345 nm of both trinitrophenylated myosins was decreased, as though the trinitrophenyl groups bound to myosin were removed. The DTT treatment also essentially reversed the inhibition of the EDTA-ATPase and Ca-ATPase activities that was caused by trinitrophenylation of myosin. These effects of trinitrophenylation and of DTT treatment were independent of the presence or absence of PP1 during the trinitrophenylation. In contrast, the PP1-induced formation of a difference spectrum of trinitrophenylated myosin was not affected by the DTT treatment. On the basis of these observations, it is suggested that the "reactive lysine residues," trinitrophenylation of which resulted in inhibition of the ATPase activities, are different from those whose trinitrophenyl groups show an altered spectrum on addition of PP1. |
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Authors:
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S Kodama; K Konno; K Arai; S Watanabe |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of biochemistry Volume: 97 ISSN: 0021-924X ISO Abbreviation: J. Biochem. Publication Date: 1985 Mar |
Date Detail:
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Created Date: 1985-09-04 Completed Date: 1985-09-04 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0376600 Medline TA: J Biochem Country: JAPAN |
Other Details:
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Languages: eng Pagination: 831-6 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphatases
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antagonists & inhibitors,
metabolism* Animals Binding Sites / drug effects Calcium-Transporting ATPases / metabolism Diphosphates / pharmacology* Dithiothreitol / pharmacology* Enzyme Activation / drug effects Muscles / metabolism Myosins / metabolism* Nitrobenzenes* Potassium Chloride / pharmacology Rabbits Trinitrobenzenesulfonic Acid* |
| Chemical | |
Reg. No./Substance:
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0/Diphosphates; 0/Nitrobenzenes; 2508-19-2/Trinitrobenzenesulfonic Acid; 3483-12-3/Dithiothreitol; 7447-40-7/Potassium Chloride; EC 3.6.1.-/Adenosine Triphosphatases; EC 3.6.1.-/EDTA-ATPase; EC 3.6.1.8/Calcium-Transporting ATPases; EC 3.6.4.1/Myosins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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