| Trimetazidine increases phospholipid turnover in ventricular myocyte. | |
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MedLine Citation:
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PMID: 9350047 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Trimetazidine (TMZ) is an anti-ischemic compound devoid of hemodynamic effects. It was recently suggested to induce cardiomyocyte protection by a mechanism involving lipid metabolism. The effects of TMZ were evaluated in rats on cardiac lipid composition, and in cultured rat cardiomyocytes on phospholipid metabolism. Rats were treated with TMZ for 4 weeks, and the fatty acid compositions were determined. Treatment with TMZ induced a significant decrease in phospholipid linoleic acid, balanced by a small increase in oleic and stearic acids. These changes were not correlated to alterations in plasma fatty acid composition. Cultured ventricular myocytes were treated with TMZ, 16 and 1 before experimentation. The time-dependent incorporation of radio labelled precursors of membrane phospholipids (3-inositol, 14C-ethanolamine, 14C-choline, 14C-arachidonic acid, 10 mumol/L) was investigated. The cells were harvested 30, 60, 105 or 150 min after precursor addition. In TMZ-cells, arachidonic acid (AA) incorporation was increased in the phospholipids, but not in other lipid fractions. This increase elicited a net increase in the total AA uptake. The incorporation of 3-inositol in the phospholipids was strongly stimulated by TMZ, although the uptake of inositol was not altered. The difference was significant within 30 min, and after 150 min the phospholipid labelling in TMZ cells was higher by 70%. A similar result was obtained with ethanolamine as precursor, which turnover increased by 50% in TMZ-treated cells. Conversely, the incorporation of choline was not significantly affected by the presence of TMZ. In conclusion TMZ appears to interfere with the metabolism of phospholipids in cardiac myocytes in a manner which could indicate an increase of membrane phospholipid turnover. |
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Authors:
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E Sentex; J P Sergiel; A Lucien; A Grynberg |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Molecular and cellular biochemistry Volume: 175 ISSN: 0300-8177 ISO Abbreviation: Mol. Cell. Biochem. Publication Date: 1997 Oct |
Date Detail:
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Created Date: 1997-12-04 Completed Date: 1997-12-04 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0364456 Medline TA: Mol Cell Biochem Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 153-62 Citation Subset: IM |
Affiliation:
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I.N.R.A., Unité de Nutrition Lipidique, Dijon, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arachidonic Acids / metabolism, pharmacology Carbon Radioisotopes / diagnostic use Cells, Cultured Chromatography, Thin Layer Ethanolamine / metabolism, pharmacology Fatty Acids / metabolism Heart Ventricles / cytology, drug effects*, metabolism Inositol / metabolism, pharmacology Phospholipids / metabolism* Rats Rats, Wistar Scintillation Counting Trimetazidine / pharmacology* Vasodilator Agents / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Arachidonic Acids; 0/Carbon Radioisotopes; 0/Fatty Acids; 0/Phospholipids; 0/Vasodilator Agents; 141-43-5/Ethanolamine; 5011-34-7/Trimetazidine; 6917-35-7/Inositol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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