Document Detail

Triiodothyronine induces lipid oxidation and mitochondrial biogenesis in rat Harderian gland.
MedLine Citation:
PMID:  23873539     Owner:  NLM     Status:  Publisher    
Rat Harderian gland (HG) is an orbital gland producing a copious lipid secretion. Recent studies indicate that its secretory activity is regulated by thyroid hormones. In the present study we found that both isoforms of the thyroid hormone receptor (TRα and TRβ) are expressed in rat HG. Although TRα is expressed at a higher level, only TRβ is regulated by T3. Because T3 induces an increase in lipid metabolism in rat HG, we investigated the effects of an animal's thyroid state on the expression levels of carnitine palmitoyltransferase-1 (A and B) and acyl-CoA oxidase (rate-limiting enzymes in mitochondrial and peroxisomal fatty acid oxidation, respectively), as well as on the mitochondrial compartment, thereby correlating mitochondrial activity and biogenesis with morphological analysis. We found that hypothyroidism decreased the expression of carnitine palmitoyltransferase-1B and acyl-CoA oxidase mRNA, whereas the administration of T3 to hypothyroid rats increased transcript levels. Respiratory parameters and catalase protein levels provided further evidence that T3 modulates mitochondrial and peroxisomal activities. Furthermore, in hypothyroid rat HG, the mitochondrial number and their total area decreased with respect to the control, whereas the average area of the individual mitochondrion did not change. However, the average area of the individual mitochondrion was reduced by approximately 50% in hypothyroid T3-treated HG, and the mitochondrial number and the total area of the mitochondrial compartment increased. The mitochondrial morphometric data correlated well with the molecular results. Indeed, hypothyroid status did not modify the expression of mitochondrial biogenesis genes, including PGC-1α, NRF1 and TFAM, whereas T3 treatment increased the expression level of these genes.
Alessandra Santillo; Lavinia Burrone; Sara Falvo; Rosalba Senese; Antonia Lanni; Gabriella Chieffi
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-7-19
Journal Detail:
Title:  The Journal of endocrinology     Volume:  -     ISSN:  1479-6805     ISO Abbreviation:  J. Endocrinol.     Publication Date:  2013 Jul 
Date Detail:
Created Date:  2013-7-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0375363     Medline TA:  J Endocrinol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
A Santillo, Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, Second University of Naples, Caserta, 81100, Italy.
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