Document Detail


Triglyceride synthesis from dihydroxyacetone phosphate and palmitate by microsomes from mammary glands of lactating mice.
MedLine Citation:
PMID:  628319     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Both di- and triglycerides were synthesized when microsomes isolated from mammary glands of lactating mice were incubated with dihydroxyacetone phosphate (DHAP), 1(-14)C)palmitate, ATP, CoASH, GSH, KF, MgC12, and NADPH. When NADH replaced NADPH, glyceride synthesis was very low. In the absence of either NADPH or NADH, DHAP was acylated to palmityl-DHAP. Since microsomes do not have glycerol 3-phosphate NAD:oxidoreductase activity , we inferred that glycerol 3-phosphate (GP) is not an intermediate in triglyceride biosynthesis from DHAP. This reductase, present in the cytosol, was active only with NADH. With the same concentration of either GP or DHAP, microsomes yielded essentially similar amounts of di- and triglycerides. Mitochondria, while capable of synthesizing palmityl-DHAP, did not produce di- and triglycerides.
Authors:
G A Rao; S Abraham
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Lipids     Volume:  13     ISSN:  0024-4201     ISO Abbreviation:  Lipids     Publication Date:  1978 Jan 
Date Detail:
Created Date:  1978-04-26     Completed Date:  1978-04-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  95-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Dihydroxyacetone / metabolism*
Mammary Glands, Animal / cytology,  metabolism*
Mice
Microsomes / metabolism*
Palmitates / metabolism*
Palmitic Acids / metabolism*
Triglycerides / biosynthesis*
Trioses / metabolism*
Weaning
Chemical
Reg. No./Substance:
0/Palmitates; 0/Palmitic Acids; 0/Triglycerides; 0/Trioses; 96-26-4/Dihydroxyacetone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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