Document Detail

Triglyceride synthesis from dihydroxyacetone phosphate and palmitate by microsomes from mammary glands of lactating mice.
MedLine Citation:
PMID:  628319     Owner:  NLM     Status:  MEDLINE    
Both di- and triglycerides were synthesized when microsomes isolated from mammary glands of lactating mice were incubated with dihydroxyacetone phosphate (DHAP), 1(-14)C)palmitate, ATP, CoASH, GSH, KF, MgC12, and NADPH. When NADH replaced NADPH, glyceride synthesis was very low. In the absence of either NADPH or NADH, DHAP was acylated to palmityl-DHAP. Since microsomes do not have glycerol 3-phosphate NAD:oxidoreductase activity , we inferred that glycerol 3-phosphate (GP) is not an intermediate in triglyceride biosynthesis from DHAP. This reductase, present in the cytosol, was active only with NADH. With the same concentration of either GP or DHAP, microsomes yielded essentially similar amounts of di- and triglycerides. Mitochondria, while capable of synthesizing palmityl-DHAP, did not produce di- and triglycerides.
G A Rao; S Abraham
Related Documents :
1464599 - P-hydroxyphenylacetate-3-hydroxylase. a two-protein component enzyme.
15014929 - Purification and characterization of an h2o-forming nadh oxidase from clostridium amino...
22375899 - Stimulus-responsive controlled release system by covalent immobilization of an enzyme i...
3755579 - Kinetic and inhibition studies on reduction of diphenyl sulfoxide by guinea pig liver a...
4330089 - Transient-kinetic studies of pig muscle lactate dehydrogenase.
19531029 - Biosynthesis and bioproduction of coenzyme q10 by yeasts and other organisms.
6714419 - High-pressure enzyme kinetics. lactate dehydrogenase in an optical cell that allows a r...
6427599 - Human placenta aldose reductase. forms sensitive and insensitive to inhibition by alres...
3032929 - Effect of succinate on mitochondrial lipid peroxidation. 2. the protective effect of su...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Lipids     Volume:  13     ISSN:  0024-4201     ISO Abbreviation:  Lipids     Publication Date:  1978 Jan 
Date Detail:
Created Date:  1978-04-26     Completed Date:  1978-04-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  95-8     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Dihydroxyacetone / metabolism*
Mammary Glands, Animal / cytology,  metabolism*
Microsomes / metabolism*
Palmitates / metabolism*
Palmitic Acids / metabolism*
Triglycerides / biosynthesis*
Trioses / metabolism*
Reg. No./Substance:
0/Palmitates; 0/Palmitic Acids; 0/Triglycerides; 0/Trioses; 96-26-4/Dihydroxyacetone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Linolenic acid deficiency: changes in fatty acid patterns in female and male rats raised on a linole...
Next Document:  Comparative intestinal and colonic absorption of [4-14C] cholesterol in the rat.