Document Detail

Trifluoromethyl ketones and methyl fluorophosphonates as inhibitors of group IV and VI phospholipases A(2): structure-function studies with vesicle, micelle, and membrane assays.
MedLine Citation:
PMID:  10446289     Owner:  NLM     Status:  MEDLINE    
A series of fatty alkyl trifluoromethyl ketones and methyl fluorophosphonates have been prepared and tested as inhibitors and inactivators of human groups IV and VI phospholipases A(2) (cPLA(2) and iPLA(2)). Compounds were analyzed with phospholipid vesicle-, detergent-phospholipid mixed-micelle-, and natural membrane-based assays, and, with few exceptions, the relative inhibitor potencies measured with the three assays were similar. Ph(CH(2))(4)COCF(3) and Ph(CH(2))(4)PO(OMe)F emerged as a potent inhibitor and inactivator, respectively, of iPLA(2), and both are poorly effective against cPLA(2). Of all 13 fatty alkyl trifluoromethyl ketones tested, the trifluoromethyl ketone analog of arachidonic acid is the most potent cPLA(2) inhibitor, and structurally similar compounds including the trifluoromethyl ketone analog of docosahexenoic acid are much poorer cPLA(2) inhibitors. Inactivation of cPLA(2) by fatty alkyl fluoromethylphosphonates is greatly promoted by binding of enzyme to the interface. The use of both vesicles and mixed micelles to assay phospholipase A(2) inhibitors and inactivators present at low mol fraction in the interface provides reliable rank order potencies of a series of compounds that correlate with their behavior in a natural membrane assay.
F Ghomashchi; R Loo; J Balsinde; F Bartoli; R Apitz-Castro; J D Clark; E A Dennis; M H Gelb
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1420     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1999 Aug 
Date Detail:
Created Date:  1999-09-28     Completed Date:  1999-09-28     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  45-56     Citation Subset:  IM    
Departments of Chemistry and Biochemistry, Box 351700, University of Washington, Seattle, WA 98195-1700, USA.
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MeSH Terms
Enzyme Inhibitors / chemistry,  pharmacology*
Ketones / chemistry,  pharmacology*
Membranes, Artificial
Phospholipases A / antagonists & inhibitors*,  classification
Phosphonic Acids / chemistry,  pharmacology*
Structure-Activity Relationship
U937 Cells
Grant Support
Reg. No./Substance:
0/Enzyme Inhibitors; 0/Ketones; 0/Liposomes; 0/Membranes, Artificial; 0/Micelles; 0/Phosphonic Acids; EC 3.1.1.-/Phospholipases A

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