Document Detail


Tridimensional structure of the Golgi apparatus of nonciliated epithelial cells of the ductuli efferentes in rat: an electron microscope stereoscopic study.
MedLine Citation:
PMID:  2825891     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The 3-dimensional structure of the Golgi apparatus has been analyzed in thin and thick sections of nonciliated epithelial cells of ductuli efferentes of rat by use of low- and high-voltage electron microscopes and a stereoscopic approach. In thick sections of tissue impregnated with osmium, the Golgi apparatus appeared at low magnification as a continuous network forming a corona at the apical pole of the nucleus. At higher magnification and in thin sections of tissue postfixed with reduced osmium and stained with lead citrate or treated to demonstrate phosphatase activity, the following structural features were observed. In the longitudinal axis of the Golgi network there were alternating compact and noncompact zones. The compact zones were composed of 6-8 flattened, poorly fenestrated saccules in close apposition to each other and forming stacks. The noncompact zones were composed of a number of highly fenestrated and slightly distended saccules, which were continuous with and bridged the saccules of the compact zones. In the cis-trans axis of the Golgi apparatus the following compartments were observed: (a) On the cis face there was a continuous osmiophilic tubular network referred to as the cis element; (b) a cis compartment composed of 3 or 4 NADPase-positive saccules perforated with pores in register forming wells that contained small vesicles; (c) a trans compartment composed of 1 or 2 TPPAse-positive elements underlying the NADPase ones, followed by 1 or 2 CMPase-positive elements that showed a flattened saccular part continuous with a network of anastomotic tubules. These tubular networks curved away from the overlying elements, giving these elements a 'peeling-off" configuration. These elements referred to as sacculotubular elements were discontinuous along the Golgi network. This compartment also included shriveled trans-tubular networks detached from the overlying sacculotubular elements and seemingly undergoing fragmentation into vesicles and tubules. The structural features of the elements of the trans compartment were indicative of continuous renewal.
Authors:
A Rambourg; Y Clermont; L Hermo; D Segretain
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biology of the cell / under the auspices of the European Cell Biology Organization     Volume:  60     ISSN:  0248-4900     ISO Abbreviation:  Biol. Cell     Publication Date:  1987  
Date Detail:
Created Date:  1988-02-20     Completed Date:  1988-02-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8108529     Medline TA:  Biol Cell     Country:  FRANCE    
Other Details:
Languages:  eng     Pagination:  103-15     Citation Subset:  IM    
Affiliation:
Département de Biologie du CEA, Saclay, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cilia / ultrastructure*
Epithelium / ultrastructure
Golgi Apparatus / enzymology,  ultrastructure*
Histocytochemistry
Lysosomes / ultrastructure
Male
Microscopy, Electron
Nucleotidases / metabolism
Rats
Testis / enzymology,  ultrastructure*
Thiamine Pyrophosphatase / metabolism
Chemical
Reg. No./Substance:
EC 3.1.3.-/2'-nucleotidase; EC 3.1.3.-/Nucleotidases; EC 3.1.3.-/cytidine-5'-monophosphatase; EC 3.6.1.-/Thiamine Pyrophosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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