Document Detail


Tridermal tumorigenesis of induced pluripotent stem cells transplanted in ischemic brain.
MedLine Citation:
PMID:  20216552     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Stroke is a major neurologic disorder. Induced pluripotent stem (iPS) cells can be produced from basically any part of patients, with high reproduction ability and pluripotency to differentiate into various types of cells, suggesting that iPS cells can provide a hopeful therapy for cell transplantation. However, transplantation of iPS cells into ischemic brain has not been reported. In this study, we showed that the iPS cells fate in a mouse model of transient middle cerebral artery occlusion (MCAO). Undifferentiated iPS cells (5 x 10(5)) were transplanted into ipsilateral striatum and cortex at 24 h after 30 mins of transient MCAO. Behavioral and histologic analyses were performed at 28 day after the cell transplantation. To our surprise, the transplanted iPS cells expanded and formed much larger tumors in mice postischemic brain than in sham-operated brain. The clinical recovery of the MCAO+iPS group was delayed as compared with the MCAO+PBS (phosphate-buffered saline) group. iPS cells formed tridermal teratoma, but could supply a great number of Dcx-positive neuroblasts and a few mature neurons in the ischemic lesion. iPS cells have a promising potential to provide neural cells after ischemic brain injury, if tumorigenesis is properly controlled.
Authors:
Hiromi Kawai; Toru Yamashita; Yasuyuki Ohta; Kentaro Deguchi; Shoko Nagotani; Xuemei Zhang; Yoshio Ikeda; Tohru Matsuura; Koji Abe
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-03-10
Journal Detail:
Title:  Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism     Volume:  30     ISSN:  1559-7016     ISO Abbreviation:  J. Cereb. Blood Flow Metab.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-08-03     Completed Date:  2010-09-07     Revised Date:  2013-05-30    
Medline Journal Info:
Nlm Unique ID:  8112566     Medline TA:  J Cereb Blood Flow Metab     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1487-93     Citation Subset:  IM    
Affiliation:
Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain / cytology,  pathology*,  surgery
Brain Ischemia / pathology,  surgery,  therapy
Cell Survival
Induced Pluripotent Stem Cells / cytology,  pathology,  transplantation*
Infarction, Middle Cerebral Artery / pathology,  surgery*,  therapy
Male
Mice
Mice, Inbred C57BL
Motor Activity
Neoplastic Processes
Neurogenesis
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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