| Trichothecin induces apoptosis of HepG2 cells via caspase-9 mediated activation of the mitochondrial death pathway. | |
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MedLine Citation:
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PMID: 22118979 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Trichothecin, one of fungal toxins which were encountered in food and in the environment, seriously threatens human and animal health. It has been shown that trichothecin changed the morphology of cellular mitochondria. However, the molecular mechanism remains unknown. Here we found that cell viability was attenuated by trichothecin. Features of apoptosis such as homosomal condensation and inter nucleosomal fragmentation were observed. In consistence with the elevated apoptosis rate, expression of anti-apoptotic protein Bcl-2 was diminished and expression of proapoptotic protein Bax was enhanced at mRNA levels. Furthermore, expression of caspase-9 and activity of caspase-3 were increased after the treatment of trichothecin. Accordingly, the mitochondrial membrane potential (∆Ψm) ai decreased in a dose-dependent manner. And Ca(2+) overload was induced by trichothecin, followed by the generation of reactive oxygen species (ROS). Collectedly, our results suggested that apoptosis induced by trichothecin is mediated by caspase-9 activation and the decrement of mitochondrial function resulted from the overloaded calcium and ROS production. |
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Authors:
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Rong Hui Du; Jiang Tao Cui; Ting Wang; Ai Hua Zhang; Ren Xiang Tan |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-11-17 |
Journal Detail:
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Title: Toxicon : official journal of the International Society on Toxinology Volume: - ISSN: 1879-3150 ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
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Created Date: 2011-11-28 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1307333 Medline TA: Toxicon Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Elsevier Ltd. All rights reserved. |
Affiliation:
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School of Medicine, Nanjing University, 22 Han Kou Road, Nanjing 210093, PR China; Institute of Functional Biomolecules, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, 22 Han Kou Road, Nanjing 210093, PR China; Jiangsu Key laboratory of Molelular Medicine, Nanjing University, 22 Han Kou Road, Nanjing 210093, PR China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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