Document Detail

Trichothecene production by Trichoderma brevicompactum.
MedLine Citation:
PMID:  16218663     Owner:  NLM     Status:  MEDLINE    
Trichoderma brevicompactum, T. viride, T. harzianum, T. atroviride, T. longibrachiatum, T. erinaceum, T. citrinoviride, and Hypocrea lutea were screened for production of trichothecenes after growth on one or several solid and liquid media. Trichothecenes were detected by liquid chromatography combined with online UV/vis spectroscopy and electrospray high-resolution mass spectrometry. T. brevicompactum produced trichodermin and/or harzianum A on all media investigated, with liquid media yielding the largest amounts. Detection of octa-2Z,4E,6E-trienedioic acid in the harzianum-A-producing strains indicated that harzianum A was synthesized directly by esterification of trichodermol with octa-2Z,4E,6E-trienedioic acid. Both the T. viride strain from which trichodermin was originally isolated and the T. harzianum strain from which harzianum A was originally isolated were shown to belong to T. brevicompactum based on four independent criteria: metabolite profiles, micromorphology, macromorphology on yeast extract sucrose agar and potato dextrose agar, and DNA sequences of the ITS1/ITS2 regions of the nuclear ribosomal DNA.
Kristian Fog Nielsen; Tom Gräfenhan; Doustmorad Zafari; Ulf Thrane
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  53     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-10-12     Completed Date:  2005-12-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  8190-6     Citation Subset:  IM    
Center for Microbial Biotechnology, BioCentrum-DTU, Technical University of Denmark.
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MeSH Terms
Chromatography, Liquid
Spectrometry, Mass, Electrospray Ionization
Trichoderma / metabolism*
Trichothecenes / biosynthesis*
Reg. No./Substance:

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