Document Detail


Triacylglycerol synthesis in goat mammary gland. Factors influencing the esterification of fatty acids synthesized de novo.
MedLine Citation:
PMID:  6743284     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
ATP alone had no effect on incorporation of fatty acids synthesized de novo and membrane-bound diacylglycerol into triacylglycerol. Combined addition of ATP and Mg2+ totally inhibits incorporation of fatty acids synthesized de novo and stimulated incorporation of membrane-bound diacylglycerol. ATP, Mg2+ and glycerol 3-phosphate stimulate incorporation of fatty acids synthesized de novo into triacylglycerol, but inhibited the incorporation of membrane-bound diacylglycerol. Diacylglycerol generated in situ was shown to be superior to diacylglycerols preloaded on the membrane as substrate for the diacylglycerol acyltransferase. A model is proposed to explain the effect of absorbed exogenous fatty acid on fatty acid synthesis de novo in goat mammary gland.
Authors:
H O Hansen; I Grunnet; J Knudsen
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Biochemical journal     Volume:  220     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1984 Jun 
Date Detail:
Created Date:  1984-08-15     Completed Date:  1984-08-15     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  521-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acyltransferases / metabolism
Adenosine Triphosphate / pharmacology
Animals
Diacylglycerol O-Acyltransferase
Diglycerides / metabolism
Esterification
Fatty Acids / biosynthesis*
Female
Glycerophosphates / pharmacology
Goats
Magnesium / pharmacology
Mammary Glands, Animal / drug effects,  metabolism*
Microsomes / drug effects,  metabolism
Palmitoyl Coenzyme A / pharmacology
Pregnancy
Triglycerides / biosynthesis*
Chemical
Reg. No./Substance:
0/Diglycerides; 0/Fatty Acids; 0/Glycerophosphates; 0/Triglycerides; 1763-10-6/Palmitoyl Coenzyme A; 56-65-5/Adenosine Triphosphate; 57-03-4/alpha-glycerophosphoric acid; 7439-95-4/Magnesium; EC 2.3.-/Acyltransferases; EC 2.3.1.20/Diacylglycerol O-Acyltransferase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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