Document Detail


Triacylglycerol synthesis in cultured rat hepatocytes. The rate-limiting role of diacylglycerol acyltransferase.
MedLine Citation:
PMID:  2737209     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The limiting role of diacylglycerol acyltransferase with respect to triacylglycerol synthesis in cultured rat hepatocytes was evaluated by following the inhibition of the overall synthetic flux by 2-bromooctanoate acting as an inhibitor of the diacylglycerol acyltransferase step. The flux-control coefficient of diacylglycerol acyltransferase in intact cultured hepatocytes amounted to 0.76 in the presence of saturating glycerol and either palmitate or oleate as the fatty acyl substrates. The flux-control coefficient of diacylglycerol acyltransferase in lysolecithin-permeabilized cultured hepatocytes amounted to 0.80 and 0.99 in the presence of saturating glycerol 3-phosphate and either palmitate or oleate as the fatty acyl substrate, respectively. Hence, triacylglycerol synthesis in liver cells under the experimental conditions employed is rate-limited by the diacylglycerol acyltransferase.
Authors:
N Mayorek; I Grinstein; J Bar-Tana
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  European journal of biochemistry / FEBS     Volume:  182     ISSN:  0014-2956     ISO Abbreviation:  Eur. J. Biochem.     Publication Date:  1989 Jun 
Date Detail:
Created Date:  1989-08-04     Completed Date:  1989-08-04     Revised Date:  2007-07-23    
Medline Journal Info:
Nlm Unique ID:  0107600     Medline TA:  Eur J Biochem     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  395-400     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Hebrew University, Hadassah Medical School, Jerusalem, Israel.
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MeSH Terms
Descriptor/Qualifier:
Acyl Coenzyme A / pharmacology
Acyltransferases / antagonists & inhibitors,  metabolism*
Animals
Cell Membrane Permeability
Cells, Cultured
Diacylglycerol O-Acyltransferase
Kinetics
Lipolysis / drug effects
Liver / enzymology*,  metabolism
Lysophosphatidylcholines
Octanoic Acids / pharmacology
Rats
Triglycerides / biosynthesis*
Chemical
Reg. No./Substance:
0/Acyl Coenzyme A; 0/Lysophosphatidylcholines; 0/Octanoic Acids; 0/Triglycerides; 2623-82-7/2-bromooctanoate; 71605-35-1/2-bromooctanoyl-coenzyme A; EC 2.3.-/Acyltransferases; EC 2.3.1.20/Diacylglycerol O-Acyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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