Document Detail

Treponema medium sp. nov., isolated from human subgingival dental plaque.
MedLine Citation:
PMID:  8995804     Owner:  NLM     Status:  MEDLINE    
A new Treponema species, for which we propose the name Treponema medium, was isolated from subgingival plaque from an adult with periodontal disease. The morphological characteristics, differential biochemical characteristics, and protein profiles on sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels of this organism are described. The guanine-plus-cytosine content of the DNA of T. medium is 51 mol%. The levels of DNA-DNA relatedness of the new species to other Treponema species, including Treponema denticola, Treponema vincentii, Treponema socranskii, Treponema pallidum, and Treponema phagedenis, are less than 30%. A phylogenetic analysis based on 16S rRNA sequences distinguished the new Treponema strain from strains belonging to previously described Treponema species. The type strain of T. medium is strain G7201.
T Umemoto; F Nakazawa; E Hoshino; K Okada; M Fukunaga; I Namikawa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of systematic bacteriology     Volume:  47     ISSN:  0020-7713     ISO Abbreviation:  Int. J. Syst. Bacteriol.     Publication Date:  1997 Jan 
Date Detail:
Created Date:  1997-02-13     Completed Date:  1997-02-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0042143     Medline TA:  Int J Syst Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  67-72     Citation Subset:  IM    
Department of Oral Microbiology, School of Dentistry, Asahi University, Gifu, Japan.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/D85437;  D85438;  M34265;  M34266;  M57737;  M57739;  M59294;  M71237;  M71238;  M88329;  X17547
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MeSH Terms
Bacterial Proteins / analysis
Bacteriological Techniques
Base Composition
Blotting, Western
Culture Media / metabolism
DNA, Bacterial / analysis
Electrophoresis, Polyacrylamide Gel
Microscopy, Electron
Molecular Sequence Data
Nucleic Acid Hybridization
RNA, Ribosomal, 16S / genetics
Sequence Homology, Nucleic Acid
Treponema / classification*,  genetics,  immunology,  ultrastructure
Reg. No./Substance:
0/Bacterial Proteins; 0/Culture Media; 0/DNA, Bacterial; 0/RNA, Ribosomal, 16S

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