Document Detail


Trehalose-producing enzymes MTSase and MTHase in Anabaena 7120 under NaCl stress.
MedLine Citation:
PMID:  18322734     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Salt tolerance, a multigenic trait, necessitates knowledge about biosynthesis and function of candidate gene(s) at the cellular level. Among the osmolytes, trehalose biosynthesis in cyanobacteria facing NaCl stress is little understood. Anabaena 7120 filaments exposed to 150 mM: NaCl fragmented and recovered on transfer to -NaCl medium with the increased heterocysts frequency (7%) over the control (4%). Cells failed to retain Na+ beyond a threshold [2.19 mM/cm3 (PCV)]. Whereas NaCl-stressed cells exhibited a marginal rise in K+ (1.1-fold) only at 30 h, for Na+ it was 130-fold at 48 h over cells in control. A time-course study (0-54 h) revealed reduction in intracellular Na+ beyond 48 h [0.80 mM/cm3 (PCV)] suggestive of ion efflux. The NaCl-stressed cells showed differential expression of maltooligosyltrehalose synthase (MTSase; EC 5.4.99.15) and maltooligosyltrehalose trehalohydrolase (MTHase; EC 3.2.1.141) depending on the time and the extent of intracellular Na+ buildup.
Authors:
Ravi K Asthana; Subhasha Nigam; Archana Maurya; Arvind M Kayastha; Sureshwar P Singh
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-03-06
Journal Detail:
Title:  Current microbiology     Volume:  56     ISSN:  0343-8651     ISO Abbreviation:  Curr. Microbiol.     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-03-21     Completed Date:  2008-07-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7808448     Medline TA:  Curr Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  429-35     Citation Subset:  IM    
Affiliation:
Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi, 221005, India. asthana.ravi@gmail.com
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MeSH Terms
Descriptor/Qualifier:
Anabaena / enzymology*
Chlorates / metabolism*
Glucosidases / metabolism*
Glucosyltransferases / metabolism*
Potassium / metabolism
Superoxide Dismutase / metabolism
Trehalose / metabolism
Chemical
Reg. No./Substance:
0/Chlorates; 7440-09-7/Potassium; 7775-09-9/sodium chlorate; 99-20-7/Trehalose; EC 1.15.1.-/superoxide dismutase 1; EC 1.15.1.1/Superoxide Dismutase; EC 2.4.1.-/Glucosyltransferases; EC 2.4.1.-/maltooligosyl trehalose synthase; EC 3.2.1.-/Glucosidases; EC 3.2.1.141/maltooligosyl trehalose trehalohydrolase

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