Document Detail


Trehalose metabolism by Bacillus popilliae.
MedLine Citation:
PMID:  4369400     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Trehalose was found to be utilized more readily than glucose for the growth of Bacillus popilliae NRRL B-2309MC. The pathway of degradation of trehalose was elucidated and found to differ from that reported for other organisms. Trehalase and trehalose phosphorylase activities could not be detected. Rather, trehalose was found to undergo phosphoenolpyruvate (PEP)-dependent phosphorylation, and the resulting trehalose 6-phosphate was cleaved by a phosphotrehalase to equimolar amounts of glucose and glucose 6-phosphate. The phosphotrehalase was purified 34-fold and shown to have a pH optimum of 6.5 to 7.0 and a K(m) for trehalose 6-phosphate of 1.8 mM. A mutant missing the phosphotrehalase failed to grow on trehalose but grew normally on other sugars. The mutant accumulated [(14)C]trehalose as [(14)C]trehalose 6-phosphate. Phosphorylation of trehalose by dialyzed extracts was at least 25 times faster with PEP than with adenosine 5'-triphosphate, and the phosphorylation activity was associated primarily with the particulate fraction. These data and the results of studies of [(14)C]trehalose uptake suggest that trehalose is transported into the cell as trehalose 6-phosphate by a PEP:sugar phosphotransferase system. Cell extracts of other strains of B. popilliae were also found to produce [(14)C]sugar phosphate from [(14)C]trehalose and to have phosphotrehalase activity.
Authors:
A Bhumiratana; R L Anderson; R N Costilow
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  119     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1974 Aug 
Date Detail:
Created Date:  1974-10-25     Completed Date:  1974-10-25     Revised Date:  2013-06-12    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  484-93     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bacillus / enzymology,  metabolism
Carbon Radioisotopes
Cell-Free System
Disaccharides / metabolism*
Fluorides / pharmacology
Glucose / metabolism
Mutation
Oxygen Consumption / drug effects
Phosphoenolpyruvate / pharmacology
Phosphoric Monoester Hydrolases / biosynthesis,  isolation & purification
Sugar Phosphates / biosynthesis
Trehalose / metabolism
Chemical
Reg. No./Substance:
0/Carbon Radioisotopes; 0/Disaccharides; 0/Fluorides; 0/Sugar Phosphates; 50-99-7/Glucose; 73-89-2/Phosphoenolpyruvate; 99-20-7/Trehalose; EC 3.1.3.-/Phosphoric Monoester Hydrolases
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