| Trehalose as an endogenous reserve in spores of the fungus Myrothecium verrucaria. | |
| | |
MedLine Citation:
|
PMID: 5892589 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Mandels, G. R. (U.S. Army Natick Laboratories, Natick, Mass.), Rasma Vitols, and Frederick W. Parrish. Trehalose as an endogenous reserve in spores of the fungus Myrothecium verrucaria. J. Bacteriol. 90:1589-1598. 1965.-Gross analysis of Myrothecium verrucaria spores showed approximately 3% fat, 33% carbohydrate, and 9.5% nitrogen. The water-soluble carbohydrates were trehalose, glucose, mannitol, and an unidentified phosphorylated compound. Water-soluble amino acids include leucine or norleucine (or both), valine, gamma-amino-n-butyric acid, beta-amino-n-butyric acid, ergothionine, glutamic acid, glutamine, glycine, aspartic acid, asparagine, cystine, and cystathionine. Ergosterol was also present. alphaalpha-Trehalose is the major reserve (20% of the dry weight), although approximately 30% of it appeared to be at the spore surface and was released by nonlethal treatment with 0.1 n HCl. Treatment with toluene or exposure to heat sufficient to kill the spores (20 min at 60 C) caused rapid liberation of all of the trehalose. Although spores could utilize exogenous trehalose with no appreciable lag, some stimulus, such as exposure to heat (10 min at 55 C), incubation with azide, or germination on exogenous substrates, was necessary to effect utilization of trehalose reserves. Spores have trehalase, but it is apparently at the spore surface, since it is inactivated by acid treatment which does not kill the spores. The metabolic pathway for utilization of trehalose is not known, but presumably it is not mediated by trehalase. The involvement of mannitol is indicated, since it tends to increase as trehalose decreases, although the changes are not quantitatively equivalent. |
| | |
Authors:
|
G R Mandels; R Vitols; F W Parrish |
Related Documents
:
|
6888509 - Effect of preservatives on the germination and growth of thermally injured fungal spores. 20396869 - Single-spore elemental analyses indicate that dipicolinic acid-deficient bacillus subti... 18661679 - Bifidobacterium longum-fermented broccoli supernatant inhibited the growth of candida a... 16765839 - Experimental studies on the growth and usnic acid production in "lichen"usnea ghattensi... 4977979 - Germination of single bacterial spores. 12790629 - Metabolite profiles and growth characteristics of rhizobium meliloti cultivated at diff... 10413529 - Glutamyl substrate-induced exposure of a free cysteine residue in the vitamin k-depende... 12828459 - The guineamides, novel cyclic depsipeptides from a papua new guinea collection of the m... 11758919 - Glycolic acid production using ethylene glycol-oxidizing microorganisms. |
Publication Detail:
|
Type: In Vitro; Journal Article |
Journal Detail:
|
Title: Journal of bacteriology Volume: 90 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 1965 Dec |
Date Detail:
|
Created Date: 1966-03-23 Completed Date: 1966-03-23 Revised Date: 2010-09-13 |
Medline Journal Info:
|
Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 1589-98 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acids Chemical Phenomena Chemistry Disaccharides / metabolism* Hot Temperature Hydrochloric Acid / pharmacology Mitosporic Fungi / metabolism* Spores |
| Chemical | |
Reg. No./Substance:
|
0/Amino Acids; 0/Disaccharides; 7647-01-0/Hydrochloric Acid |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Thyrocalcitonin: inhibitor of bone resorption in tissue culture.
Next Document: Specificity of the heme requirement for growth of Bacteroides ruminicola.