Document Detail

Trehalose-6P synthase is essential for trehalase activation triggered by glucose, nitrogen source or heat shock, but not by osmostress, in Schizosaccharomyces pombe.
MedLine Citation:
PMID:  9729425     Owner:  NLM     Status:  MEDLINE    
Cells of Schizosaccharomyces pombe disrupted in the tps1+ gene, which encodes trehalose-6P synthase, were unable to increase trehalase activity in response to the addition of glucose or nitrogen source. Moreover, in contrast to normal cells, Deltatps1 cells did not increase trehalase activity by heat shock. Overexpression of tps1+ in cells devoid of trehalose-6P synthase restored the ability to increase trehalase after addition of nutrients or by heat shock. In glucose-repressed cells, which are normally refractory to the activation of trehalase by glucose, overexpression of tps1+ enabled the cells to increase trehalase activity upon addition of the sugar. Northern hybridisations were used to determine the level of mRNA for trehalase in normal and Deltatps1 cells. Transcription for trehalase was not significantly altered upon addition of glucose or nitrogen source, but increased markedly in heat-shocked cells even though trehalase activity remained unchanged in Deltatps1 cells. These findings provide evidence for a role of trehalose-6P synthase in the signalling pathway causing post-transcriptional activation of neutral trehalase induced by nutrients or heat shock. However, trehalase increased in Deltatps1 cells under hypertonic conditions suggesting the existence in Schiz. pombe of a distinct regulatory mechanism for enhancement of trehalase, specifically triggered by osmostress.
J Cansado; J Vicente-Soler; T Soto; J Fernandez; M Gacto
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1381     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1998 Aug 
Date Detail:
Created Date:  1998-10-01     Completed Date:  1998-10-01     Revised Date:  2010-01-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  271-8     Citation Subset:  IM    
Copyright Information:
Copyright Elsevier Science B.V.
Department of Genetics and Microbiology, University of Murcia, 30071 Murcia, Spain.
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MeSH Terms
Enzyme Activation / drug effects
Gene Expression
Glucose / pharmacology*
Glucosyltransferases / genetics,  metabolism*
Hot Temperature*
Nitrogen / pharmacology*
Osmolar Concentration
RNA, Messenger / metabolism
Schizosaccharomyces / enzymology*
Trehalase / metabolism*
Reg. No./Substance:
0/RNA, Messenger; 50-99-7/Glucose; 7727-37-9/Nitrogen; EC 2.4.1.-/Glucosyltransferases; EC synthase; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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