Document Detail

Trefoil factor family-peptides promote migration of human bronchial epithelial cells: synergistic effect with epidermal growth factor.
MedLine Citation:
PMID:  11694446     Owner:  NLM     Status:  MEDLINE    
A process termed "restitution" enables rapid repair of the respiratory epithelium by migration of neighbouring cells. Mucin-associated TFF-peptides (formerly P-domain peptides or trefoil factors) are typical motogens enhancing migration of cells in various in vitro models mimicking restitution of the intestine. The human bronchial epithelial cell line BEAS-2B was used as a model system of airway restitution. The motogenic activities of recombinant human TFF2 as well as porcine TFF2 were demonstrated by in vitro wound healing assays of BEAS-2B cells. TFF2 did not induce phosphorylation of the epidermal growth factor (EGF) receptor. EGF was capable of enhancing the motogenic effect of human TFF2 at a concentration of 3 x 10(-10) M whereas EGF itself (i.e., in the absence of TFF2) did not stimulate migration at this low concentration. Furthermore, TFF2 as well as monomeric and dimeric forms of TFF3 enhanced migration of BEAS-2B cells in Boyden chambers. Motogenic activity of TFF2 was also shown for normal human bronchial epithelial (NHBE) cells in Boyden chambers. These results suggest that TFF-peptides act as motogens in the human respiratory epithelium triggering rapid repair of damaged mucosa in the course of airway diseases such as asthma.
M Oertel; A Graness; L Thim; F Bühling; H Kalbacher; W Hoffmann
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of respiratory cell and molecular biology     Volume:  25     ISSN:  1044-1549     ISO Abbreviation:  Am. J. Respir. Cell Mol. Biol.     Publication Date:  2001 Oct 
Date Detail:
Created Date:  2001-11-05     Completed Date:  2001-12-31     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8917225     Medline TA:  Am J Respir Cell Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  418-24     Citation Subset:  IM    
Institut für Molekularbiologie und Medizinische Chemie, Otto-von-Guericke-Universität, Leipziger Strasse 44, D-39120 Magdeburg, Germany.
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MeSH Terms
Bronchi / cytology*,  drug effects
Cell Culture Techniques / methods
Cell Movement / drug effects
Cells, Cultured
Epidermal Growth Factor / pharmacology
Growth Substances / pharmacology*
Muscle Proteins*
Peptides / pharmacology*
Proteins / pharmacology*
Receptor, Epidermal Growth Factor / drug effects,  metabolism
Respiratory Mucosa / cytology*,  drug effects
Wounds and Injuries / pathology
Reg. No./Substance:
0/Growth Substances; 0/Mucins; 0/Muscle Proteins; 0/Neuropeptides; 0/Peptides; 0/Proteins; 0/TFF3 protein, human; 0/TFF3 protein, rat; 146046-78-8/trefoil factor; 62229-50-9/Epidermal Growth Factor; EC, Epidermal Growth Factor
Comment In:
Am J Respir Cell Mol Biol. 2001 Oct;25(4):401-4   [PMID:  11694443 ]

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