Document Detail

Trefoil factor 2 (TFF2) deficiency in murine digestive tract influences the immune system.
MedLine Citation:
PMID:  16121031     Owner:  NLM     Status:  MEDLINE    
BACKGROUND AND AIMS: The gastrointestinal trefoil factor family (TFF1, TFF2, TFF3) peptides are considered to play an important role in maintaining the integrity of the mucosa. The physiological role of TFF2 in the protection of the GI tract was investigated in TFF2 deficiency. METHODS: TFF2-/- mice were generated and differential expression of various genes was assessed by using a mouse expression microarray, quantitative real time PCR, Northern blots or immunohistochemistry. RESULTS: On an mRNA level we found 128 differentially expressed genes. We observed modulation of a number of crucial genes involved in innate and adaptive immunity in the TFF2-/- mice. Expression of proteasomal subunits genes (LMP2, LMP7 and PSMB5) involved in the MHC class I presentation pathway were modulated indicating the formation of immunoproteasomes improving antigen presentation. Expression of one subunit of a transporter (TAP1) responsible for importing degraded antigens into ER was increased, similarly to the BAG2 gene that modulates chaperone activity in ER helping proper loading on MHC class I molecules. Several mouse defensin (cryptdin) genes coding important intestinal microbicidal proteins were up-regulated as a consequence of TFF2 deficiency. Normally moderate expression of TFF3 was highly increased in stomach.
Mirela Baus-Loncar; Janinne Schmid; El-Nasir Lalani; Ian Rosewell; Robert A Goodlad; Gordon W H Stamp; Nikolaus Blin; Tuncay Kayademir
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology     Volume:  16     ISSN:  1015-8987     ISO Abbreviation:  Cell. Physiol. Biochem.     Publication Date:  2005  
Date Detail:
Created Date:  2005-08-25     Completed Date:  2005-11-02     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9113221     Medline TA:  Cell Physiol Biochem     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  31-42     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2005 S. Karger AG, Basel.
Division of Molecular Genetics, Institute of Human Genetics, University of Tuebingen, Germany.
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MeSH Terms
Antigen Presentation
Base Sequence
DNA Primers / genetics
Digestive System / immunology*,  metabolism*
Gene Expression Profiling
Gene Targeting
Histocompatibility Antigens Class I / metabolism
Immunity, Innate
Mice, Inbred C57BL
Mice, Knockout
Mucins / deficiency*,  genetics,  immunology
Muscle Proteins / deficiency*,  genetics,  immunology
Oligonucleotide Array Sequence Analysis
Peptides / deficiency*,  genetics,  immunology
Proteasome Endopeptidase Complex / genetics
Protein Precursors / genetics
Reg. No./Substance:
0/DNA Primers; 0/Histocompatibility Antigens Class I; 0/Mucins; 0/Muscle Proteins; 0/Peptides; 0/Protein Precursors; 0/TFF2 protein, mouse; 120668-29-3/cryptdin; EC Endopeptidase Complex

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