Document Detail


Treatment with antisense oligonucleotide reduces the expression of type I collagen in a human-skin organ-wound model: implications for antifibrotic gene therapy.
MedLine Citation:
PMID:  18046156     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Increased collagen expression during wound healing causes scar formation, abnormal contracture, low tensile strength, functional impairment, and disfigurement. A novel ex vivo wound-injury model demonstrated that AS60, an antisense oligonucleotide (ASO) to type I collagen, reduced the mRNA and protein expression of type 1 collagen. Following a cutaneous wound injury in a human-skin organ culture, AS60 injection resulted in a 36% (P < 0.001) and 30% decrease (P < 0.001) in type 1 collagen mRNA and protein expression after 7 days. Similarly, transfection of cultured human fibroblasts with ASO resulted in a 36% decrease (P < 0.001) and a 31% decrease (P < 0.001) in type 1 collagen mRNA and protein expression. Immunofluorescence of human skin organ culture treated with ASO showed a specific reduction in collagen expression. Using AS60 to reduce collagen expression in human skin may have implications for its use as a gene therapy agent to reduce the formation of fibrotic scarring.
Authors:
Rahul K Nath; Weijun Xiong; Andrea D Humphries; Rajinder Beri
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Annals of plastic surgery     Volume:  59     ISSN:  0148-7043     ISO Abbreviation:  Ann Plast Surg     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2007-11-29     Completed Date:  2008-02-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7805336     Medline TA:  Ann Plast Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  699-706     Citation Subset:  IM    
Affiliation:
Texas Nerve and Paralysis Institute, Houston, TX 77030, USA. nath@drnathmedical.com
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MeSH Terms
Descriptor/Qualifier:
Blotting, Northern
Blotting, Western
Cells, Cultured
Collagen Type I / genetics*
Fibroblasts / drug effects*,  pathology
Fibrosis / genetics*,  therapy*
Gene Therapy / methods*
Humans
Immunohistochemistry
Oligoribonucleotides, Antisense / pharmacology*,  therapeutic use*
Skin Diseases / pathology,  therapy*
Wounds and Injuries / therapy*
Chemical
Reg. No./Substance:
0/Collagen Type I; 0/Oligoribonucleotides, Antisense

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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