Document Detail

Treatment with anti-NAP monoclonal antibody reduces disease severity in murine model of novel angiogenic protein-induced or ovalbumin-induced arthritis.
MedLine Citation:
PMID:  23286942     Owner:  NLM     Status:  MEDLINE    
Rheumatoid arthritis (RA) is a polyarticular inflammatory, angiogenic disease. Synovial angiogenesis contributes to inflammation in RA. In this study we have developed an arthritic model in rats using a novel angiogenic protein (NAP), isolated from human synovial fluid of RA patients. We produced anti-NAP monoclonal antibodies (mAbs) and investigated the therapeutic efficacy of the same in adjuvant-induced or NAP-induced arthritis as a model of human RA. The treatment of arthritic rats with anti-NAP mAbs resulted in effective amelioration of paw oedema, radiological arthritic characteristics, serum levels of vascular endothelial growth factor (VEGF) and NAP, compared to that of untreated arthritic animals. Further, profiling of angiogenic markers such as synovial microvessel density, angiogenesis, CD31, VEGF and fms-like tyrosine kinase (Flt1) by immunohistochemistry both in arthritic and anti-NAP mAb-treated animals revealed the efficacy of mAb as an anti-angiogenic functional antibody. Therefore, NAP may be an attractive target to design anti-angiogenic and anti-arthritic therapies to control the pathogenesis of arthritis.
N B Nataraj; J Krishnamurthy; B P Salimath
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical and experimental immunology     Volume:  171     ISSN:  1365-2249     ISO Abbreviation:  Clin. Exp. Immunol.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-04     Completed Date:  2013-03-12     Revised Date:  2014-02-04    
Medline Journal Info:
Nlm Unique ID:  0057202     Medline TA:  Clin Exp Immunol     Country:  England    
Other Details:
Languages:  eng     Pagination:  155-63     Citation Subset:  IM    
Copyright Information:
© 2012 British Society for Immunology.
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MeSH Terms
Angiogenesis Inducing Agents / immunology,  isolation & purification
Antibodies, Monoclonal / therapeutic use*
Arthritis, Experimental / immunology,  therapy*
Arthritis, Rheumatoid / immunology,  therapy*
Calcium-Binding Proteins / immunology
Cells, Cultured
Disease Models, Animal
Disease Progression
Immunotherapy / methods*
Membrane Proteins / immunology
Middle Aged
Ovalbumin / immunology
Rats, Wistar
Synovial Membrane / drug effects,  immunology
Reg. No./Substance:
0/Angiogenesis Inducing Agents; 0/Antibodies, Monoclonal; 0/Calcium-Binding Proteins; 0/Membrane Proteins; 9006-59-1/Ovalbumin

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