Document Detail


Treatment with anti-LFA-1 alpha monoclonal antibody selectively interferes with the maturation of CD4- 8+ thymocytes.
MedLine Citation:
PMID:  9176108     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Maturation of T lymphocytes in the thymus is driven by signals provided by soluble factors and by the direct interaction between thymocytes and stromal cells. Although the interaction between T-cell receptor (TCR) and major histocompalibility complex (MHC) molecules on stromal cells is crucial for T-cell development, other accessory molecules seem to play a role in this process. In order to better understand the role of lymphocyte function-associated antigen-1 (LFA-1) and intercellular adhesion molecule-1 (ICAM-1) molecules in thymocyte maturation, mice were treated from birth with saturating doses of non-cytolytic-specific monoclonal antibodies. The effect of this treatment on thymocyte subpopulations and the expression of CD3 and TCR-alpha beta by these cells was investigated by flow cytometry. Our data demonstrated that the effective saturation of LFA-1 alpha chain in the thymus, but not ICAM-I or LFA-I beta chain, selectively interfered with the maturation of CD8+ T cells, as manifested by a marked reduction in the frequency of CD4-8+ thymocytes expressing high levels of CD3 and TCR-alpha beta. This selective reduction was also observed in peripheral blood mononuclear cells and spleen cells. The analysis of the frequencies of various V beta TCR showed that CD4-8+ thymocytes were globally affected by the treatment. These results underline the importance of the interaction between LFA-1 and its ligands in the maturation of CD8+ T cells and document the existence of different molecular requirements for the differentiation of CD4+ and CD8+ T cells.
Authors:
C Revilla; A L González; C Conde; M López-Hoyos; J Merino
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Immunology     Volume:  90     ISSN:  0019-2805     ISO Abbreviation:  Immunology     Publication Date:  1997 Apr 
Date Detail:
Created Date:  1997-06-23     Completed Date:  1997-06-23     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0374672     Medline TA:  Immunology     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  550-6     Citation Subset:  IM; X    
Affiliation:
Sección de Inmunologia, Hospital Universitario Marqués de Valdecilla, Instituto Nacional de la Salud, Santander, Spain.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / immunology
Antigens, CD3 / metabolism
CD4-Positive T-Lymphocytes / immunology
CD8-Positive T-Lymphocytes / immunology*
Cell Differentiation / immunology
Intercellular Adhesion Molecule-1 / immunology
Lymphocyte Count
Lymphocyte Function-Associated Antigen-1 / immunology*
Mice
Mice, Inbred BALB C
Mice, Inbred Strains
Receptors, Antigen, T-Cell, alpha-beta / metabolism
Thymus Gland / immunology*
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, CD3; 0/Lymphocyte Function-Associated Antigen-1; 0/Receptors, Antigen, T-Cell, alpha-beta; 126547-89-5/Intercellular Adhesion Molecule-1
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