| Treatment with Qing'E, a kidney-invigorating Chinese herbal formula, antagonizes the estrogen decline in ovariectomized mice. | |
| | |
MedLine Citation:
|
PMID: 20707715 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A Chinese herbal preparation, Qing'E formula (QEF), has been used clinically for treating osteoporosis in postmenopausal women by virtue of its kidney-invigorating function; however, no evidence base links QEF to estrogen replacement therapy. In this study, we undertake a characterization of estrogenic activity of QEF using an in vivo model of ovariectomized (OVX) mice together with in vitro studies with the MCF-7 cells for further molecular characterization. OVX mice were treated intragastrically with QEF at doses of 0.85, 1.7, and 3.4 g/kg per day for 4 weeks. QEF treatments restored the estrus cycle and demonstrated significant estrogenic activity, as indicated by reversal of uterine atrophy (six-fold increase in uterine weight), reduction in rectal temperature, and increased expression (1.6-fold) of estrogen receptors (ERs) in the uterus. Notably, the largest changes in these three parameters were found at the lowest dose. At the highest dose of QEF, significant changes were found in adrenal gland weight (30% increase), serum estradiol (E(2)) (60% increase), and luteinizing hormone (LH) (17% decrease) compared with untreated OVX controls. The data suggest estrogenic responses induced by QEF show tissue variation that reflects different affinities of ERs for QEF components. QEF could significantly induce luciferase expression (2.7-fold compared with control) from an estrogen response element luciferase reporter and induced expression of ERalpha and ERbeta (1.2-fold and 1.7-fold respectively) in MCF-7 cells. Both activities were inhibited 80-90% by the estrogen antagonist ICI 182,780. This study demonstrates that QEF activity is mediated through estrogenic components and provides an evidence base for QEF treatment of postmenopausal symptoms. |
| | |
Authors:
|
Ying Xu; Zi-Jia Zhang; Fang Geng; Shi-bing Su; Kenneth N White; S W Annie Bligh; Christopher J Branford-White; Zheng-tao Wang |
Related Documents
:
|
17003025 - Genetics and hormones in testicular descent. 22968785 - Inhibition of neu-induced mammary carcinogenesis in transgenic mice expressing erΔ3, a... 18719025 - Estrogen actions in the male reproductive system involve estrogen response element-inde... 21743025 - Role of the fas/fasl system in a model of rsv infection in mechanically ventilated mice. 14725965 - Association between inflammation and nigral neuronal damage following striatal excitoto... 19549245 - Immune evasion during varicella zoster virus infection of keratinocytes. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Rejuvenation research Volume: 13 ISSN: 1557-8577 ISO Abbreviation: Rejuvenation Res Publication Date: 2010 Aug |
Date Detail:
|
Created Date: 2010-08-25 Completed Date: 2010-12-02 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101213381 Medline TA: Rejuvenation Res Country: United States |
Other Details:
|
Languages: eng Pagination: 479-88 Citation Subset: IM |
Affiliation:
|
The MOE Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adrenal Glands
/
drug effects Animals Cell Line, Tumor Dose-Response Relationship, Drug Drugs, Chinese Herbal* Estradiol / blood* Estrus / drug effects Female Humans Immunohistochemistry Luteinizing Hormone / metabolism Mice Organ Size / drug effects Ovariectomy* Receptors, Estrogen / metabolism Uterus / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Drugs, Chinese Herbal; 0/Receptors, Estrogen; 50-28-2/Estradiol; 9002-67-9/Luteinizing Hormone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Acinetobacter baumannii 2002-2008: increase of carbapenem-associated multiclass resistance in the Un...
Next Document: Immuno-positron emission tomography: shedding light on clinical antibody therapy.