Document Detail

Treatment with a histone deacetylase inhibitor after nuclear transfer improves the preimplantation development of cloned bovine embryos.
MedLine Citation:
PMID:  20962457     Owner:  NLM     Status:  MEDLINE    
We examined the effects of treatment with histone deacetylase inhibitors (HDACi), trichostatin A (TSA) and scriptaid (SCR), on the blastocyst formation rate in bovine somatic cell nuclear transferred (SCNT) embryos derived from fibroblast cells. Three fibroblast cell lines (L1, L2 and L3) were used as somatic cell donors to produce SCNT embryos (L1, L2 and L3 embryos, respectively). In Experiment 1, we compared the in vitro developmental competence of L1 embryos treated with various concentrations of TSA for different time periods following chemical activation. Embryos treated with 5 nM TSA for 20 h showed a significantly increased blastocyst formation rate compared with untreated controls. In Experiment 2, we examined the effect of TSA (5 nM) treatment of L1, L2 and L3 embryos as well as the effect of treatment of L1, L2 and L3 embryos with various concentrations of SCR on in vitro developmental competence. It was found that 5 nM TSA treatment significantly increased the blastocyst formation rate in L1 and L3 embryos but did not have an influence on the development of L2 embryos. On the other hand, 5 nM SCR treatment significantly increased the blastocyst formation rates of L1 and L2 embryos compared with controls. However, there was no significant increase in the blastocyst formation rate of L3 embryos when they were treated with SCR. In Experiment 3, acetylation of H4K12 was examined in donor cells and pronuclear-stage L1, L2 and L3 embryos treated with 5 nM TSA or 5 nM SCR by immunostaining. The level of H4K12 acetylation was different among donor cells. The staining intensities in the TSA-treated L1 and L3 embryos and SCR-treated L2 embryos were significantly higher than those of untreated embryos. These results suggest that HDACi treatment of bovine SCNT embryos improves the blastocyst formation rate; however, the optimal treatment conditions may differ among donor cell lines.
Satoshi Akagi; Kazutsugu Matsukawa; Eiji Mizutani; Kazuhiro Fukunari; Masahiro Kaneda; Shinya Watanabe; Seiya Takahashi
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Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2010-10-16
Journal Detail:
Title:  The Journal of reproduction and development     Volume:  57     ISSN:  1348-4400     ISO Abbreviation:  J. Reprod. Dev.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-03-22     Completed Date:  2011-08-04     Revised Date:  2011-12-13    
Medline Journal Info:
Nlm Unique ID:  9438792     Medline TA:  J Reprod Dev     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  120-6     Citation Subset:  IM    
Reproductive Biology and Technology Research Team, National Institute of Livestock and Grassland Science, NARO, Ibaraki, Japan.
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MeSH Terms
Acetylation / drug effects
Blastocyst / cytology,  drug effects*,  physiology*
Cell Line
Cloning, Organism / methods*
Ectogenesis / drug effects*
Embryo Culture Techniques
Histone Deacetylase Inhibitors / pharmacology*
Histones / metabolism
Hydroxamic Acids / pharmacology
Hydroxylamines / pharmacology
Nuclear Transfer Techniques
Osmolar Concentration
Quinolines / pharmacology
Time Factors
Reg. No./Substance:
0/Histone Deacetylase Inhibitors; 0/Histones; 0/Hydroxamic Acids; 0/Hydroxylamines; 0/Quinolines; 0/scriptaid; 58880-19-6/trichostatin A

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