| Treatment of skin defects using suspensions of in vitro cultured keratinocytes. | |
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MedLine Citation:
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PMID: 8198724 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The in vitro cultivation of keratinocytes and their application in the form of confluent sheets to cover various kinds of skin defects involves a number of problematical steps which could be improved by using single cell suspensions instead. Therefore we developed a method to apply keratinocytes suspended as single cells in a fibrin gel. By testing the feasibility of this method in different experimental animal models we found that it facilitates cultivation as well as application of the cells, moreover, this method allows a much more flexible use of the cells, i.e. it is easier to consider the clinical condition of the patient than by the conventional method. |
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Authors:
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B Hafemann; R Hettich; S Ensslen; B Kowol; A Zühlke; R Ebert; M Königs; C J Kirkpatrick |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Burns : journal of the International Society for Burn Injuries Volume: 20 ISSN: 0305-4179 ISO Abbreviation: Burns Publication Date: 1994 Apr |
Date Detail:
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Created Date: 1994-07-07 Completed Date: 1994-07-07 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8913178 Medline TA: Burns Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 168-72 Citation Subset: IM |
Affiliation:
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Clinic of Burns, Plastic and Reconstructive Surgery, Klinikum of the RWTH Aachen, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cells, Cultured Female Keratinocytes / cytology, transplantation* Male Rats Rats, Inbred Lew Rats, Inbred Strains Skin / pathology Skin Transplantation* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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