Document Detail


Treatment of reperfused ischemia with adipose-derived stem cells in a preclinical swine model of myocardial infarction.
MedLine Citation:
PMID:  22524986     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
AIMS: To determine the long-term effect of transplantation of adipose-derived stromal cells (ADSCs) in a preclinical model of ischemia/reperfusion (I/R).METHODS AND RESULTS: I/R was induced in 28 Goettingen minipigs by 120 minutes coronary artery occlusion followed by reperfusion. Nine days later, surviving animals were allocated to receive trans-endocardial injection of a mean of 213.6±41.78 million green fluorescent protein (GFP)-expressing ADSCs (n=7) or culture medium as control (n=9). Heart function, cell engraftment and histological analysis were performed 3 months after transplantation. Transplantation of ADSCs induced a statistically significant long-lasting (3 months) improvement in cardiac function and geometry in comparison with control animals.Functional improvement was associated with an increase in angiogenesis and vasculogenesis and a positive effect on heart remodeling with a decrease in fibrosis and cardiac hypertrophy in animals treated with ADSCs. Despite the lack of cell engraftment after 3 months, ADSC transplantation induced changes in the ratio between MMP/TIMP.CONCLUSION: Our results indicate that transplantation of ADSCs, despite the lack of longterm significant cell engraftment, increases vessel density and prevents adverse remodeling in a clinically relevant model of myocardial infarction, strongly suggesting a paracrine mediated effect. ADSCs thus constitute an attractive candidate for the treatment of myocardial infarction.
Authors:
Manuel Mazo; Salomón Hernández; Juan José Gavira; Gloria Abizanda; Miriam Araña; Tania López-Martínez; Cristina Moreno; Juana Merino; Alba Martino-Rodríguez; Alicia Uixeira; José A García de Jalón; Juan Pastrana; Diego Martínez-Caro; Felipe Prosper
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-17
Journal Detail:
Title:  Cell transplantation     Volume:  -     ISSN:  1555-3892     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-4-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9208854     Medline TA:  Cell Transplant     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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