Document Detail


Treatment of rat proximal and distal colonic cells with sodium orthovanadate enhances their adhesion and survival in primary culture.
MedLine Citation:
PMID:  9243806     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have studied the effect of sodium orthovanadate, an inhibitor of protein tyrosine phosphatases, on primary cultures of colonocytes and stromal cells. Everted proximal and distal colonic tissue of adult rats were disintegrated by a collagenase/dispase solution for 60 min at 37 degrees C to prepare viable gland fragments and isolated cells. Cell preparations were inoculated onto plastic substratum or cytodex-3 microcarriers in a defined maintenance medium or in 1% fetal calf serum media. Incorporation of sodium orthovanadate (> or = 50 microM) in these media constantly enhanced the survival (cell enumeration and trypan blue exclusion P < 0.05) and the adhesion (up to four-fold by crystal violet staining, P < 0.01) of colonocytes (characterized by cytokeratin-18, transforming growth factor-alpha or alkaline phosphatase expression) and stromal cells. Removal of sodium orthovanadate from culture media restored cellular death processes. Incorporation of 10 mM n-butyric acid did not promote cell adhesion and survival except for distal cells exposed to 2 mM sodium orthovanadate. Besides studies in the regulation of anoikis in primary culture, the model will help to assay the influences of dietary and growth factors on the biology of non-cancerous colonic cells.
Authors:
B Kaeffer; C Bénard; H M Blottière; C Cherbut
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell biology international     Volume:  21     ISSN:  1065-6995     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  1997 May 
Date Detail:
Created Date:  1997-09-09     Completed Date:  1997-09-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  303-14     Citation Subset:  IM    
Affiliation:
CRNH de Nantes, INRA, Laboratoire de Technologie Appliquée à la Nutrition, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Anticoagulants / pharmacology
Butyric Acid
Butyric Acids / pharmacology
Cell Adhesion / drug effects
Cell Division / drug effects
Cell Survival / drug effects
Cells, Cultured
Colon / cytology*
Epithelial Cells
Heparin / pharmacology
Histamine Antagonists / pharmacology
Rats
Stromal Cells / cytology
Vanadates / pharmacology*
Chemical
Reg. No./Substance:
0/Anticoagulants; 0/Butyric Acids; 0/Histamine Antagonists; 0/Vanadates; 107-92-6/Butyric Acid; 9005-49-6/Heparin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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