Document Detail

Treatment of Vicia faba root tip cells with specific inhibitors to cyclin-dependent kinases leads to abnormal spindle formation.
MedLine Citation:
PMID:  10069076     Owner:  NLM     Status:  MEDLINE    
Many events during cell division are triggered by an evolutionary conserved regulator, the cyclin-dependent kinase (Cdk). Here we used two novel drugs, the purine analogues bohemine and roscovitine, to study the role of Cdks in cell cycle progression and microtubule organisation in Vicia faba root tip cells. Both drugs inhibited the activity of immunopurified Vicia faba and alfalfa Cdc2-kinase. The transcript levels of an A- and B-type cyclin, as well as of the cdc2 genes, declined in treated root tips, while the mRNA level of a D-type cyclin gene was not affected. An observed transient arrest at the G1/S and G2/M regulatory points indicated that inhibition of the Cdc2-kinase had an effect on both transitions. In contrast to the regular bipolar spindle in untreated cell, in drug-treated metaphase cells abnormally short and dense kinetochore microtubule fibres were observed. These microtubules were randomly arranged in the vicinity of the kinetochores and connected the chromosomes. Thus, the chromosomes were not aligned on the metaphase plate but were arranged in a circle, with kinetochores pointing inwards and chromosome arms pointing outwards. gamma-Tubulin, which plays a role in microtubule nucleation, also localised to the centre of the monopolar spindle. The observed abnormalities in mitosis, after inhibition of Cdc2-kinase by specific drugs, suggest a role for this enzyme in regulating some of the steps leading to a bipolar spindle structure.
P Binarová; J Dolezel; P Draber; E Heberle-Bors; M Strnad; L Bögre
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Plant journal : for cell and molecular biology     Volume:  16     ISSN:  0960-7412     ISO Abbreviation:  Plant J.     Publication Date:  1998 Dec 
Date Detail:
Created Date:  1999-04-01     Completed Date:  1999-04-01     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9207397     Medline TA:  Plant J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  697-707     Citation Subset:  IM    
Norman Borlaug Center for Plant Science De Montfort University, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
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MeSH Terms
CDC2 Protein Kinase / antagonists & inhibitors,  genetics*
Calcium-Calmodulin-Dependent Protein Kinases / metabolism
Cell Cycle / drug effects
Cyclin A / genetics
Cyclin B / genetics
Enzyme Inhibitors / pharmacology
Fabaceae / cytology*
Meristem / cytology,  drug effects,  ultrastructure
Microtubules / drug effects,  ultrastructure*
Mitotic Spindle Apparatus / drug effects*
Plant Roots / cytology,  drug effects*,  ultrastructure
Plants, Medicinal*
Protein Kinases / metabolism
Purines / pharmacology*
Transcription, Genetic
Reg. No./Substance:
0/Cyclin A; 0/Cyclin B; 0/Enzyme Inhibitors; 0/Purines; 0/bohemine; 0/roscovitine; EC 2.7.-/Protein Kinases; EC Protein Kinases; EC Protein Kinase

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