| Treatment of Nuclear-Donor Cells or Cloned Zygotes with Chromatin-Modifying Agents Increases Histone Acetylation But Does Not Improve Full-Term Development of Cloned Cattle. | |
| | |
MedLine Citation:
|
PMID: 22468998 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Abstract Although somatic cell nuclear transfer (SCNT) is a promising tool, its potential use is hampered by the high mortality rates during the development to term of cloned offspring. Abnormal epigenetic reprogramming of donor nuclei after SCNT is thought to be the main cause of this low efficiency. We hypothesized that chromatin-modifying agents (CMAs) targeting chromatin acetylation and DNA methylation could alter the chromatin configuration and turn them more amenable to reprogramming. Thus, bovine fibroblasts were treated with 5-aza-2'-deoxycytidine (AZA) plus trichostatin (TSA) or hydralazine (HH) plus valproic acid (VPA) whereas, in another trial, cloned bovine zygotes were treated with TSA. The treatment of fibroblasts with either AZA+TSA or HH+VPA increased histone acetylation, but did not affect the level of DNA methylation. However, treatment with HH+VPA decreased cellular viability and proliferation. The use of these cells as nuclear donors showed no positive effect on pre- and postimplantation development. Regarding the treatment of cloned zygotes with TSA, treated one-cell embryos showed an increase in the acetylation patterns, but not in the level of DNA methylation. Moreover, this treatment revealed no positive effect on pre- and postimplantation development. This work provides evidence the treatment of either nuclear donor cells or cloned zygotes with CMAs has no positive effect on pre- and postimplantation development of cloned cattle. |
| | |
Authors:
|
Juliano Rodrigues Sangalli; Tiago Henrique Camara De Bem; Felipe Perecin; Marcos Roberto Chiaratti; Lilian de Jesus Oliveira; Reno Roldi de Araújo; José Rodrigo Valim Pimentel; Lawrence Charles Smith; Flávio Vieira Meirelles |
Related Documents
:
|
236548 - Size fractionation of double-stranded dna by precipitation with polyethylene glycol. 16878128 - Sulfation patterns of glycosaminoglycans encode molecular recognition and activity. 1031838 - Studies on the bacteriophage ps8 of agrobacterium tumefaciens (smith and townsend) conn... 6974728 - Haemophilus influenzae polypeptides involved in deoxyribonucleic acid uptake detected b... 21787748 - Accumulation of p21 proteins at dna damage sites independent of p53 and core nhej facto... 6262918 - 5-methylcytosine in eukaryotic dna. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-4-2 |
Journal Detail:
|
Title: Cellular reprogramming Volume: - ISSN: 2152-4998 ISO Abbreviation: - Publication Date: 2012 Apr |
Date Detail:
|
Created Date: 2012-4-3 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101528176 Medline TA: Cell Reprogram Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
1 Departamento de Ciências Básicas, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo , Pirassununga, São Paulo, Brazil . |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Early aberrations in chromatin dynamics in embryos produced under in vitro conditions.
Next Document: Discovery and Optimization of a Novel Spiro-pyrrolidine Inhibitors of beta-Secretase (BACE1) Through...