Document Detail

Treatment of fungal keratitis from Fusarium infection by corneal cross-linking.
MedLine Citation:
PMID:  22081155     Owner:  NLM     Status:  MEDLINE    
PURPOSE: To evaluate the efficacy of corneal cross-linking (CXL) (riboflavin-UV-A) as a simple therapy in Fusarium keratitis.
METHODS: Twenty-four rabbits were systemically anesthetized, and the stromata of their right corneas were inoculated with Fusarium solani [10(5) colony-forming units (CFU) per milliliter]. Rabbits were divided into 2 groups: one was treated with CXL 72 hours after infection and the other did not receive any treatment (control). All eyes in both the groups were examined before (days 0 and 3) and after (day 7) CXL treatment. The eyes were enucleated, and corneal buttons were sent for microbiological and histological examinations.
RESULTS: All animals developed Fusarium keratitis; there was no statistically significant difference between groups before treatment (day 0, P = 0.397 and day 3, P = 0.702). After CXL treatment, the difference in clinical scores on day 7 between groups was statistically significant (P = 0.00); the CXL group showed significant lower clinical score. The CXL group had 22.45 ± 5.09 CFU/g compared with 42.5 ± 3.12 CFU/g in the control group; this difference was statistically significant (P = 0.01). In the 3 buttons of the control group, similar amounts of Fusarium hyphae and inflammatory cells were observed. In 2 of the 3 buttons analyzed from the CXL group, fewer Fusarium hyphae, inflammatory cells, and nonspecific stromal changes were observed compared with the control group.
CONCLUSIONS: Treatment of fungal keratitis with CXL seems to be effective in decreasing the intensity and severity of infectious keratitis by F. solani. This therapy may be useful as a coadjuvant in the medical treatment of resistant infections.
Gustavo Galperin; Martín Berra; Julia Tau; Gabriela Boscaro; Jorge Zarate; Alejandro Berra
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cornea     Volume:  31     ISSN:  1536-4798     ISO Abbreviation:  Cornea     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-01-16     Completed Date:  2012-03-06     Revised Date:  2013-02-19    
Medline Journal Info:
Nlm Unique ID:  8216186     Medline TA:  Cornea     Country:  United States    
Other Details:
Languages:  eng     Pagination:  176-80     Citation Subset:  IM    
Laboratorio de Investigaciones Oculares, Buenos Aires, Argentina.
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MeSH Terms
Colony Count, Microbial
Disease Models, Animal
Eye Infections, Fungal / microbiology,  radiotherapy*
Fusariosis / microbiology,  radiotherapy*
Fusarium / isolation & purification
Keratitis / microbiology,  radiotherapy*
Photosensitizing Agents / therapeutic use
Riboflavin / therapeutic use
Ultraviolet Therapy*
Reg. No./Substance:
0/Photosensitizing Agents; 83-88-5/Riboflavin
Comment In:
Cornea. 2013 Feb;32(2):218   [PMID:  23172118 ]
Cornea. 2013 Feb;32(2):217-8   [PMID:  23086375 ]

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