| Treatment of C6 glioma cells and rats with antidepressant drugs increases the detergent extraction of G(s alpha) from plasma membrane. | |
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MedLine Citation:
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PMID: 10461902 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Results from previous studies suggested that chronic treatment of rats or C6 glioma cells with antidepressants augments the coupling between Gs and adenylyl cyclase. As these effects on C6 glioma cells are seen in the absence of presynaptic input, several antidepressant drugs may have a direct "postsynaptic" effect on their target cells. It was hypothesized that the target of antidepressant action was some membrane protein that may regulate coupling between G proteins and adenylyl cyclase. To test this, C6 glioma cells were treated with amitriptyline, desipramine, iprindole, or fluoxetine for 3 days. Chlorpromazine served as a control for these treatments. Membrane proteins were extracted sequentially with Triton X-100 and Triton X-114 from C6 glioma cells. Triton X-100 extracted more G(s alpha) in membranes prepared from antidepressant-treated C6 glioma cells than from control groups. In addition, cell fractionation studies revealed that the amount of G(s alpha) in caveolin-enriched domains was reduced after antidepressant treatment and that adenylyl cyclase comigrated with G(s alpha) in the gradients. These data suggest that some postsynaptic component that increases availability of Gs to activate effector molecules, such as adenylyl cyclase, might be a target of antidepressant treatment. |
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Authors:
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S Toki; R J Donati; M M Rasenick |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of neurochemistry Volume: 73 ISSN: 0022-3042 ISO Abbreviation: J. Neurochem. Publication Date: 1999 Sep |
Date Detail:
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Created Date: 1999-09-14 Completed Date: 1999-09-14 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 2985190R Medline TA: J Neurochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1114-20 Citation Subset: IM |
Affiliation:
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Department of Physiology and Biophysics, University of Illinois College of Medicine, Chicago 60612-7342, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenylate Cyclase
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metabolism Animals Antidepressive Agents / pharmacology* Antidepressive Agents, Tricyclic / pharmacology Cell Membrane / chemistry, drug effects, metabolism Centrifugation, Density Gradient Desipramine / pharmacology Detergents GTP-Binding Protein alpha Subunits, Gs / biosynthesis*, chemistry, isolation & purification Glioma Humans Male Octoxynol Polyethylene Glycols Rats Rats, Sprague-Dawley Solubility Sucrose Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
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HL 07692-09/HL/NHLBI NIH HHS; MH39595/MH/NIMH NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antidepressive Agents; 0/Antidepressive Agents, Tricyclic; 0/Detergents; 0/Polyethylene Glycols; 50-47-5/Desipramine; 57-50-1/Sucrose; 9002-93-1/Octoxynol; 9036-19-5/Nonidet P-40; EC 3.6.5.1/GTP-Binding Protein alpha Subunits, Gs; EC 4.6.1.1/Adenylate Cyclase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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