|Treating fat grafts with human endothelial progenitor cells promotes their vascularization and improves their survival in diabetes mellitus.|
|PMID: 23018694 Owner: NLM Status: MEDLINE|
|BACKGROUND: Bone marrow-derived endothelial progenitor cells are required for vascularization of a fat graft to form a functional microvasculature within the graft and to facilitate its integration into the surrounding tissues. Organ transplantation carries a high risk of graft loss and rejection in patients with diabetes mellitus because endothelial progenitor cell function is impaired. The authors investigated the influence of endothelial progenitor cell treatment on the phenotype and survival of human fat grafts in immunocompromised mice with experimentally induced diabetes mellitus.
METHODS: The authors injected 1 ml of human fat tissue into the scalps of 14 nondiabetic and 28 diabetic immunocompromised mice, and then treated some of the grafts with endothelial progenitor cells that was isolated from the blood of a human donor. The phenotype of the endothelial progenitor cell-treated fat grafts from the 14 diabetic mice was compared with that of the untreated fat grafts from 14 nondiabetic and 14 diabetic mice, 18 days and 15 weeks after fat transplantation. Determination of graft phenotype included measurements of weight and volume, vascular endothelial growth factor levels, vascular endothelial growth factor receptor-2, endothelial nitric oxide synthase, and caspase 3 expression levels, and histologic analysis of the extent of vascularization.
RESULTS: The untreated grafts from the diabetic mice were fully resorbed 15 weeks after fat transplantation. The phenotype of endothelial progenitor cell-treated fat grafts from the diabetic mice was similar to that of the untreated fat grafts from the nondiabetic mice.
CONCLUSION: Endothelial progenitor cell treatment of transplanted fat can increase the survival of a fat graft by inducing its vascularization and decreasing the extent of apoptosis.
|Saher Hamed; Ohad Ben-Nun; Dana Egozi; Aviad Keren; Nastya Malyarova; Danny Kruchevsky; Amos Gilhar; Yehuda Ullmann|
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|Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't|
|Title: Plastic and reconstructive surgery Volume: 130 ISSN: 1529-4242 ISO Abbreviation: Plast. Reconstr. Surg. Publication Date: 2012 Oct|
|Created Date: 2012-09-28 Completed Date: 2012-12-14 Revised Date: 2014-10-13|
Medline Journal Info:
|Nlm Unique ID: 1306050 Medline TA: Plast Reconstr Surg Country: United States|
|Languages: eng Pagination: 801-11 Citation Subset: AIM; IM|
|APA/MLA Format Download EndNote Download BibTex|
Bone Marrow Transplantation
Diabetes Mellitus, Experimental / immunology
Disease Models, Animal
Graft Rejection / prevention & control
Neovascularization, Physiologic / physiology*
Sensitivity and Specificity
Stem Cell Transplantation / methods*
Streptozocin / pharmacology
Tissue Transplantation / adverse effects, methods
Tissue and Organ Harvesting
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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