Document Detail

Treadmill exercise in claudicants on aspirin results in improved antioxidant status but only minimal platelet activation.
MedLine Citation:
PMID:  16287611     Owner:  NLM     Status:  MEDLINE    
The consequence of exercise on platelets remains controversial and adverse effects may result from repeated ischaemia reperfusion injury. We investigated platelet activation (platelet P-selectin (PS), and activated glycoprotein (Gp) IIb/IIIa), platelet-monocyte aggregates (PMA) and total plasma antioxidant status (TPAS) in claudicants after exercise. Twenty claudicants, taking 75 mg of aspirin daily, were subjected to repeated treadmill testing (3 km/h, 10% inclination). Blood was sampled before and after exercise. Activated GpIIb/IIIa, PS and PMA were quantified with flow cytometry. TPAS was quantified using a decolourisation assay. Percent positive cells for PS (pre-exercise 3.76% vs. 40 min post-exercise 4.10%; P < 0.05) and platelet-monocyte aggregates (pre-exercise: 25.31% vs. 40 min post-exercise 26.99%; P < 0.05) were significantly higher after exercise. Relative median fluorescence (RMF) for activated GpIIb/IIIa was significantly higher 40 min after exercise (pre-exercise: 3.04 vs. 40 min post-exercise: 4.01; P < 0.05). TPAS was significantly higher post-exercise (pre-exercise: 1.31 mmol/l vs. 1 min post-exercise: 1.40 mmol/l and 40 min post-exercise: 1.38 mmol/l; P < 0.01). Following moderate exercise, 'aspirin treated claudicants' show marginal platelet activation, PMA formation and a favourable improvement in antioxidant status. Further studies are required to assess the effect of additional antiplatelet agents and the significance of platelet-monocyte interactions. The possibility that aspirin contributes to the TPAS changes following exercise needs to be investigated.
M Cleanthis; J Smout; V Bhattacharya; H Ashour; B Shenton; G Stansby
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Platelets     Volume:  16     ISSN:  0953-7104     ISO Abbreviation:  Platelets     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-11-18     Completed Date:  2006-01-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9208117     Medline TA:  Platelets     Country:  England    
Other Details:
Languages:  eng     Pagination:  446-52     Citation Subset:  IM    
Department of Vascular Surgery, Queen Elizabeth II Hospital, Gateshead, UK.
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MeSH Terms
Aged, 80 and over
Antioxidants / analysis
Aspirin / administration & dosage*
Case-Control Studies
Exercise Test* / adverse effects
Intermittent Claudication / blood*,  drug therapy
Middle Aged
Oxidation-Reduction / drug effects
P-Selectin / blood
Plant Extracts
Platelet Aggregation / drug effects*
Platelet Aggregation Inhibitors / administration & dosage*
Platelet Glycoprotein GPIIb-IIIa Complex / analysis
Reperfusion Injury / blood*,  etiology
Reg. No./Substance:
0/Antioxidants; 0/P-Selectin; 0/Phenols; 0/Plant Extracts; 0/Platelet Aggregation Inhibitors; 0/Platelet Glycoprotein GPIIb-IIIa Complex; 0/antioxidant biofactor AOB; 50-78-2/Aspirin

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