Document Detail

Traumatic injury in vitro induces IEG mRNAs in cultured glial cells, suppressed by co-culture with neurons.
MedLine Citation:
PMID:  10574349     Owner:  NLM     Status:  MEDLINE    
Glial changes following traumatic injury to glial monolayers as well as to neuronal-glial co-culture systems in vitro were examined with a focus on the expression of mRNAs coding for the immediate early genes (IEG) c-fos, c-jun and zif/268, demonstrated using in situ hybridization. Glial cells along scratch wound lines extended cytoplasmic processes as early as 10 min post-injury and the whole wound was covered with gliosis by 24 h. For complete restoration in the case of glial cells co-cultured with neurons, this required 48 h. Induction of the three IEG mRNAs was eminent along the edges of scratch wound, peaking at 30-60 min post-injury and subsiding by 3 h. The peak expression of IEG mRNAs was delayed to 1-3 h post-injury and became undetectable at 6 h in neuronal-glial co-cultures. The data suggest that mechanical injury to glial cells causes gliosis and the expression of IEG mRNAs, which are suppressed by co-culture with neurons, indicating some influence of neuronal-glial interactions.
H Katano; K Fujita; T Kato; K Asai; Y Kawamura; A Masago; K Yamada
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Neuroreport     Volume:  10     ISSN:  0959-4965     ISO Abbreviation:  Neuroreport     Publication Date:  1999 Aug 
Date Detail:
Created Date:  1999-12-21     Completed Date:  1999-12-21     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9100935     Medline TA:  Neuroreport     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  2439-48     Citation Subset:  IM    
Department of Neurosurgery, Nagoya City University Medical School, Nagoya, Japan.
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MeSH Terms
Brain Injuries / metabolism*,  pathology
Cells, Cultured
Coculture Techniques
Genes, Immediate-Early*
Genes, fos
Genes, jun
In Situ Hybridization
Neuroglia / metabolism*,  pathology
Neurons / metabolism*,  pathology
RNA, Messenger / biosynthesis*
Rats, Wistar
Reg. No./Substance:
0/RNA, Messenger

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