| Traumatic injury in vitro induces IEG mRNAs in cultured glial cells, suppressed by co-culture with neurons. | |
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MedLine Citation:
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PMID: 10574349 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Glial changes following traumatic injury to glial monolayers as well as to neuronal-glial co-culture systems in vitro were examined with a focus on the expression of mRNAs coding for the immediate early genes (IEG) c-fos, c-jun and zif/268, demonstrated using in situ hybridization. Glial cells along scratch wound lines extended cytoplasmic processes as early as 10 min post-injury and the whole wound was covered with gliosis by 24 h. For complete restoration in the case of glial cells co-cultured with neurons, this required 48 h. Induction of the three IEG mRNAs was eminent along the edges of scratch wound, peaking at 30-60 min post-injury and subsiding by 3 h. The peak expression of IEG mRNAs was delayed to 1-3 h post-injury and became undetectable at 6 h in neuronal-glial co-cultures. The data suggest that mechanical injury to glial cells causes gliosis and the expression of IEG mRNAs, which are suppressed by co-culture with neurons, indicating some influence of neuronal-glial interactions. |
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Authors:
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H Katano; K Fujita; T Kato; K Asai; Y Kawamura; A Masago; K Yamada |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Neuroreport Volume: 10 ISSN: 0959-4965 ISO Abbreviation: Neuroreport Publication Date: 1999 Aug |
Date Detail:
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Created Date: 1999-12-21 Completed Date: 1999-12-21 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 9100935 Medline TA: Neuroreport Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 2439-48 Citation Subset: IM |
Affiliation:
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Department of Neurosurgery, Nagoya City University Medical School, Nagoya, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain Injuries / metabolism*, pathology Cells, Cultured Coculture Techniques Genes, Immediate-Early* Genes, fos Genes, jun In Situ Hybridization Neuroglia / metabolism*, pathology Neurons / metabolism*, pathology RNA, Messenger / biosynthesis* Rats Rats, Wistar |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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