Document Detail

Traumatic brain injury induces macrophage subsets in the brain.
MedLine Citation:
PMID:  23630120     Owner:  NLM     Status:  MEDLINE    
Traumatic brain injury (TBI) elicits innate inflammatory responses that can lead to secondary brain injury. To better understand the mechanisms involved in TBI-induced inflammation, we examined the nature of macrophages responding to TBI in mice. In this model, brain macrophages were increased >20-fold the day after injury and >77-fold 4 days after injury in the ipsilateral hemisphere compared with sham controls. TBI macrophage subsets were identified by using a reporter mouse strain (YARG) that expresses eYFP from an internal ribosome entry site (IRES) inserted at the 3' end of the gene for arginase-1 (Arg1), a hallmark of alternatively activated (M2) macrophages. One day after TBI, 21 ± 1.5% of ipsilateral brain macrophages expressed relatively high levels of Arg1 as detected by yellow fluorescent protein, and this subpopulation declined thereafter. Arg1(+) cells localized with macrophages near the TBI lesion. Gene expression analysis of sorted Arg1(+) and Arg1(-) brain macrophages revealed that both populations had profiles that included features of conventional M2 macrophages and classically activated (M1) macrophages. The Arg1(+) cells differed from Arg1(-) cells in multiple aspects, most notably in their chemokine repertoires. Thus, the macrophage response to TBI initially involves heterogeneous polarization toward at least two major subsets.
Christine L Hsieh; Charles C Kim; Bryan E Ryba; Erene C Niemi; Jennifer K Bando; Richard M Locksley; Jialing Liu; Mary C Nakamura; William E Seaman
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2013-06-05
Journal Detail:
Title:  European journal of immunology     Volume:  43     ISSN:  1521-4141     ISO Abbreviation:  Eur. J. Immunol.     Publication Date:  2013 Aug 
Date Detail:
Created Date:  2013-08-09     Completed Date:  2013-10-31     Revised Date:  2014-10-30    
Medline Journal Info:
Nlm Unique ID:  1273201     Medline TA:  Eur J Immunol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  2010-22     Citation Subset:  IM    
Copyright Information:
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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MeSH Terms
Arginase / genetics,  metabolism*
Bacterial Proteins / genetics
Brain / immunology*
Brain Injuries / immunology*
Cell Movement
Chemokines / biosynthesis
Gene Expression Profiling
Inflammation / immunology
Luminescent Proteins / genetics
Macrophage Activation / immunology*
Macrophages / immunology*
Mice, Inbred C57BL
Mice, Transgenic
Ribosomes / genetics,  metabolism
Grant Support
Reg. No./Substance:
0/Bacterial Proteins; 0/Chemokines; 0/Luminescent Proteins; 0/yellow fluorescent protein, Bacteria; EC protein, mouse; EC

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