Document Detail


Trap profiles of projector based optoelectronic tweezers (OET) with HeLa cells.
MedLine Citation:
PMID:  19333286     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this paper we present trap profile measurements for HeLa cells in Optoelectronic Tweezers (OET) based on a data projector. The data projector is used as a light source to illuminate amorphous Si creating virtual electrodes which are used to trap particles through dielectrophoresis. We show that although the trap stiffness is typically greater at the edges of the optical spot it is possible to create a trap with constant trap stiffness by reducing the trap's size until it is similar to the object being trapped. We have successfully created a trap for HeLa cells with a constant trap stiffness of 3 x 10(-6) Nm-1 (capable of moving the cell up to 50 microms-1) with a 12 microm diameter trap. We also calculate the depth of the potential well that the cell will experience due to the trap and find that it to be 1.6 x 10(-16)J (4 x 10(4) kBT).
Authors:
Steven L Neale; Aaron T Ohta; Hsan-Yin Hsu; Justin K Valley; Arash Jamshidi; Ming C Wu
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Optics express     Volume:  17     ISSN:  1094-4087     ISO Abbreviation:  Opt Express     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-03-31     Completed Date:  2009-06-02     Revised Date:  2013-10-30    
Medline Journal Info:
Nlm Unique ID:  101137103     Medline TA:  Opt Express     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5232-9     Citation Subset:  IM    
Affiliation:
Department of Electrical Engineering and Computer Sciences, Berkeley Sensor and Actuator Center, University of California, Berkeley, CA 94720, USA.sln2@eecs.berkeley.edu
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MeSH Terms
Descriptor/Qualifier:
Computer-Aided Design
Electronics / instrumentation*
Electrophoresis / instrumentation*,  methods
Equipment Design
Equipment Failure Analysis
HeLa Cells
Humans
Lighting / instrumentation*
Micromanipulation / instrumentation*,  methods
Optical Devices*
Optical Tweezers*
Reproducibility of Results
Sensitivity and Specificity
Grant Support
ID/Acronym/Agency:
#PN2 EY018228/EY/NEI NIH HHS; PN2 EY018228/EY/NEI NIH HHS; PN2 EY018228-01/EY/NEI NIH HHS
Comments/Corrections

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