Document Detail

Transthyretin Aggregates Induce Production of Reactive Nitrogen Species.
MedLine Citation:
PMID:  22627469     Owner:  NLM     Status:  Publisher    
Background and Objective: Misfolded and aggregated transthyretins (agTTR) contribute to neurodegenerative amyloid diseases such as familial amyloid polyneuropathy and senile systemic amyloidosis. The neurotoxicity mechanisms of agTTR, however, are not well understood. In the current study, the possible contribution of reactive nitrogen species (RNS) to such mechanisms was investigated by examining agTTR-mediated changes in cellular RNS levels. Methods and Results: The production of RNS was assessed through nitrate and nitrite assays in two human cell lines after exposure to agTTR (2.4 μM pre-aggregation concentration). In both epidermoid (A431) and schwannoma (sNF94.3) cell lines, agTTR induced significant increases in RNS (p < 0.05 relative to the same concentration of normal TTR, or no-TTR controls). Redox modulators such as apocynin (1-(4-hydroxy-3-methoxy-phenyl)ethanone) and L-NMMA (N(G)-monomethyl-L-arginine) were tested for their effects on RNS production. These modulators decreased RNS production in both cell lines; although the effects of L-NMMA were statistically significant only in the schwannoma cells. Moreover, cells treated with agTTR exhibited decreases in metabolic activity relative to TTR- or non-TTR-treated cells (p < 0.05) as assessed by reduction of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide). Conclusion: The results provide novel evidence for involvement of RNS in pro-oxidative effects of agTTR in two different human cell lines, and show that agTTR can induce more generalized changes in cellular metabolic activity.
Vai-Hong Fong; Amandio Vieira
Related Documents :
22729369 - Zoledronic acid inhibits human nasopharyngeal carcinoma cell proliferation by activatin...
6952709 - Discrimination between primary pleural and primary peritoneal mesotheliomas by morphome...
22819839 - Glucagon-like peptide 1 receptor plays an essential role in geniposide attenuating lipo...
3091949 - Improved detection of terminal transferase (tdt): the use of detergents on glutaraldehy...
8539239 - Recognition of atypical reserve cell hyperplasia in cervical smears and its diagnostic ...
22851869 - Pi3k expression and pik3ca mutations are related to colorectal cancer metastases.
11559769 - Heterogeneous expression of the delayed-rectifier k+ currents i(k,r) and i(k,s) in rabb...
4075529 - Normal pyruvate oxidation in friedreich ataxia and charcot-marie-tooth disease fibrobla...
2926909 - Cytotoxicity of high energy shock waves: methodologic considerations.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-5-24
Journal Detail:
Title:  Neuro-degenerative diseases     Volume:  -     ISSN:  1660-2862     ISO Abbreviation:  -     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-5-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101189034     Medline TA:  Neurodegener Dis     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 S. Karger AG, Basel.
Department of Biomedical Physiology and Kinesiology, Simon Fraser University, Burnaby, B.C., Canada.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Development of a Carbon-14 Labeling Approach to Support Disposition Studies with a Pegylated Biologi...
Next Document:  Changes in von Willebrand factor-cleaving protease (ADAMTS-13) in patients with aortic stenosis unde...