| Transsynaptic tracing with vesicular stomatitis virus reveals novel retinal circuitry. | |
| | |
MedLine Citation:
|
PMID: 23283320 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
The use of neurotropic viruses as transsynaptic tracers was first described in the 1960s, but only recently have such viruses gained popularity as a method for labeling neural circuits. The development of retrograde monosynaptic tracing vectors has enabled visualization of the presynaptic sources onto defined sets of postsynaptic neurons. Here, we describe the first application of a novel viral tracer, based on vesicular stomatitis virus (VSV), which directs retrograde transsynaptic viral spread between defined cell types. We use this virus in the mouse retina to show connectivity between starburst amacrine cells (SACs) and their known synaptic partners, direction-selective retinal ganglion cells, as well as to discover previously unknown connectivity between SACs and other retinal ganglion cell types. These novel connections were confirmed using physiological recordings. VSV transsynaptic tracing enables cell type-specific dissection of neural circuitry and can reveal synaptic relationships among neurons that are otherwise obscured due to the complexity and density of neuropil. |
| | |
Authors:
|
Kevin T Beier; Bart G Borghuis; Rana N El-Danaf; Andrew D Huberman; Jonathan B Demb; Constance L Cepko |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: The Journal of neuroscience : the official journal of the Society for Neuroscience Volume: 33 ISSN: 1529-2401 ISO Abbreviation: J. Neurosci. Publication Date: 2013 Jan |
Date Detail:
|
Created Date: 2013-01-03 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8102140 Medline TA: J Neurosci Country: United States |
Other Details:
|
Languages: eng Pagination: 35-51 Citation Subset: IM |
Affiliation:
|
Departments of Genetics and Ophthalmology, Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115, Departments of Ophthalmology and Visual Science and Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06510, and Departments of Neurosciences, Ophthalmology, and Neurobiology Section in the Division of Biology, University of California, San Diego, La Jolla, California 92093. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Periadolescent exposure to the NMDA receptor antagonist MK-801 impairs the functional maturation of ...
Next Document: Signals for previous goal choice persist in the dorsomedial, but not dorsolateral striatum of rats.