Document Detail

Transsynaptic tracing with vesicular stomatitis virus reveals novel retinal circuitry.
MedLine Citation:
PMID:  23283320     Owner:  NLM     Status:  MEDLINE    
The use of neurotropic viruses as transsynaptic tracers was first described in the 1960s, but only recently have such viruses gained popularity as a method for labeling neural circuits. The development of retrograde monosynaptic tracing vectors has enabled visualization of the presynaptic sources onto defined sets of postsynaptic neurons. Here, we describe the first application of a novel viral tracer, based on vesicular stomatitis virus (VSV), which directs retrograde transsynaptic viral spread between defined cell types. We use this virus in the mouse retina to show connectivity between starburst amacrine cells (SACs) and their known synaptic partners, direction-selective retinal ganglion cells, as well as to discover previously unknown connectivity between SACs and other retinal ganglion cell types. These novel connections were confirmed using physiological recordings. VSV transsynaptic tracing enables cell type-specific dissection of neural circuitry and can reveal synaptic relationships among neurons that are otherwise obscured due to the complexity and density of neuropil.
Kevin T Beier; Bart G Borghuis; Rana N El-Danaf; Andrew D Huberman; Jonathan B Demb; Constance L Cepko
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of neuroscience : the official journal of the Society for Neuroscience     Volume:  33     ISSN:  1529-2401     ISO Abbreviation:  J. Neurosci.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-01-03     Completed Date:  2013-03-12     Revised Date:  2014-08-21    
Medline Journal Info:
Nlm Unique ID:  8102140     Medline TA:  J Neurosci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  35-51     Citation Subset:  IM    
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MeSH Terms
Nerve Net / drug effects,  physiology*
Neuronal Tract-Tracers / pharmacology*
Neurons / drug effects,  physiology*
Retina / drug effects,  physiology*
Synapses / drug effects,  physiology*
Grant Support
NS068012-01/NS/NINDS NIH HHS; P30 HD018655/HD/NICHD NIH HHS; R01 EY014454/EY/NEI NIH HHS; R01 EY014454/EY/NEI NIH HHS; R01 EY022157-01/EY/NEI NIH HHS; //Howard Hughes Medical Institute; //Howard Hughes Medical Institute
Reg. No./Substance:
0/Neuronal Tract-Tracers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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